Asian Journal of Bio Science

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In Vitro Micropropagation of Gerbera Using Auxillary Bud


Affiliations
1 Plant Tissue Culture Division, Jain Irrigation Systems Ltd., Jain Hills, Jalgaon, M.S., India
     

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A protocol for rapid clonal micropropagation of gerbera was developed by auxillary bud.MS+BAP 2.0+NAA 0.5 mg/l, which was given early bud initiation (19.40 days), with higher initiation per cent (91.66%) along with longer shoot length (2.5 cm). MS+BAP 2.0+NAA 0.5+Ads 100 mg/ l produced higher number of shoots (7.33 shoots/explants). The micro shoots were ischolar_mained (96%) in just 8.56 days on MS+NAA 2.0+activated charcoal 750 mg/l. In vitro ischolar_mained plants were acclimatized on sand + soil + FYM+ leaf mould. Plants shows 82.43 per cent survival rate during acclimatization. The plants were established in the field after acclimatization.

Keywords

Gerbera, Auxillary Bud, Micropropagation
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  • In Vitro Micropropagation of Gerbera Using Auxillary Bud

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Authors

A. R. Kadu
Plant Tissue Culture Division, Jain Irrigation Systems Ltd., Jain Hills, Jalgaon, M.S., India

Abstract


A protocol for rapid clonal micropropagation of gerbera was developed by auxillary bud.MS+BAP 2.0+NAA 0.5 mg/l, which was given early bud initiation (19.40 days), with higher initiation per cent (91.66%) along with longer shoot length (2.5 cm). MS+BAP 2.0+NAA 0.5+Ads 100 mg/ l produced higher number of shoots (7.33 shoots/explants). The micro shoots were ischolar_mained (96%) in just 8.56 days on MS+NAA 2.0+activated charcoal 750 mg/l. In vitro ischolar_mained plants were acclimatized on sand + soil + FYM+ leaf mould. Plants shows 82.43 per cent survival rate during acclimatization. The plants were established in the field after acclimatization.

Keywords


Gerbera, Auxillary Bud, Micropropagation

References