Asian Journal of Bio Science

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Studies on Polyphenol Oxidase in Pomegranate (Punica granatum L.)


Affiliations
1 Department of Biochemistry and Molecular Biology, College of Agricultural Biotechnology, Loni (ITI Campus) Rahata, Ahmednagar (M.S.), India
     

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The present investigation was undertaken to study properties of polyphenol oxidase and monitor changes in polyphenal oxidase activity in Ganesh cultivar of pomegranate during storage. The characterization of polyphenol oxidase in pomegranate fruit extract with respect to effect of enzyme concentration and substrate concentration was studied. For studying changes in polyphenol oxidase activity during storage, the pomegranate fruits at half yellow stage were freshly harvested and stored at ambient temperature and analyzed after 0, 2, 4, 6, 8 and 10 days of storage. The polyphenol oxidase activity has increased up to 8th day of storage and thereafter, decreased slightly on 10th day.

Keywords

Ambient, Half Yellow Stage, Substrate, Enzyme
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  • E1, Nemar, S.E., Ismail, I.A. and Rogab, N. (1990). Chemical composition of juice and seed of pomegranate fruits. Die Natrunt, 34(7): 601-606.

  • Haplin and Lee (1987). Formula to determine the enzyme activity.

  • Im, J.S, Parkin, K.L. and Van Elbe, J.H. (1990). Endogenous ployphenol oxidase activity associated with blackening defects in canned beet (Beta vulgaris L) ischolar_main slice. J. Food Sci., 55(4): 1043-1045.

  • Jaiswal, Vidhan, DerMarderosiana, Ara and Portera, John R. (2010). Anthocyanins and polyphenol oxidase from dried arils of pomegranate (Punica granatum L.). Food Chem., 118 (1) : 11-16

  • Joslyn, M.A. and Ponting, J.D. (1951). Ascorbic acid oxidation and browning in apple tissue. Arch. Biochem. Bio phys., 19: 47-63

  • Kumar, K.B. and Khan, P.A. (1982). peroxidase and polyphenol oxidase in excised ragi leaves during senescence. Indian J. Bxpt. Biol., 20(5): 412-4.

  • Lowry, O.H., Rosebrough, N.J, Farr, A.L. and Randall, R.J. (1951). Protein measurement with folin phenol reagents, J. Blol. Chem., 19(3): 265-275.

  • Mayar, A.M. and Harel, E. (1981). Polyphenol oxidase In plant in Recent Advances In the Biochemistry of Fruits and Vegetable. Academic Press. New York. pp.161-180.

  • Mote, U.N, Ajri, D.S. and Tarobe, A.B. (1992). Anannotated list of pests infesting pomegranate in Central Maharashtrs. Man. J. Hort., 6(1): 96-98.

  • Panse, V.G. and Sukhatme, P.V. (1967). Statistical methods for agricultural workers. Indian Council of Agricultural Research, New Delhi.

  • Purohit, A.J. (1986). Pomegranate. Plantation Crops. Opportunities Constrains, Vol. II. Oxford and IBH. Publilshing Co. Pvt. New Delhi, ppx 273-281.


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  • Studies on Polyphenol Oxidase in Pomegranate (Punica granatum L.)

Abstract Views: 376  |  PDF Views: 0

Authors

M.R. Shelke
Department of Biochemistry and Molecular Biology, College of Agricultural Biotechnology, Loni (ITI Campus) Rahata, Ahmednagar (M.S.), India
S.A. Fargade
Department of Biochemistry and Molecular Biology, College of Agricultural Biotechnology, Loni (ITI Campus) Rahata, Ahmednagar (M.S.), India
R.V. Darade
Department of Biochemistry and Molecular Biology, College of Agricultural Biotechnology, Loni (ITI Campus) Rahata, Ahmednagar (M.S.), India

Abstract


The present investigation was undertaken to study properties of polyphenol oxidase and monitor changes in polyphenal oxidase activity in Ganesh cultivar of pomegranate during storage. The characterization of polyphenol oxidase in pomegranate fruit extract with respect to effect of enzyme concentration and substrate concentration was studied. For studying changes in polyphenol oxidase activity during storage, the pomegranate fruits at half yellow stage were freshly harvested and stored at ambient temperature and analyzed after 0, 2, 4, 6, 8 and 10 days of storage. The polyphenol oxidase activity has increased up to 8th day of storage and thereafter, decreased slightly on 10th day.

Keywords


Ambient, Half Yellow Stage, Substrate, Enzyme

References