Asian Journal of Bio Science

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Studies on Polyphenol Oxidase in Pomegranate (Punica granatum L.)


Affiliations
1 Department of Biochemistry and Molecular Biology, College of Agricultural Biotechnology, Loni (ITI Campus) Rahata, Ahmednagar (M.S.), India
     

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The present investigation was undertaken to study properties of polyphenol oxidase and monitor changes in polyphenal oxidase activity in Ganesh cultivar of pomegranate during storage. The characterization of polyphenol oxidase in pomegranate fruit extract with respect to effect of enzyme concentration and substrate concentration was studied. For studying changes in polyphenol oxidase activity during storage, the pomegranate fruits at half yellow stage were freshly harvested and stored at ambient temperature and analyzed after 0, 2, 4, 6, 8 and 10 days of storage. The polyphenol oxidase activity has increased up to 8th day of storage and thereafter, decreased slightly on 10th day.

Keywords

Ambient, Half Yellow Stage, Substrate, Enzyme
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  • Studies on Polyphenol Oxidase in Pomegranate (Punica granatum L.)

Abstract Views: 377  |  PDF Views: 0

Authors

M.R. Shelke
Department of Biochemistry and Molecular Biology, College of Agricultural Biotechnology, Loni (ITI Campus) Rahata, Ahmednagar (M.S.), India
S.A. Fargade
Department of Biochemistry and Molecular Biology, College of Agricultural Biotechnology, Loni (ITI Campus) Rahata, Ahmednagar (M.S.), India
R.V. Darade
Department of Biochemistry and Molecular Biology, College of Agricultural Biotechnology, Loni (ITI Campus) Rahata, Ahmednagar (M.S.), India

Abstract


The present investigation was undertaken to study properties of polyphenol oxidase and monitor changes in polyphenal oxidase activity in Ganesh cultivar of pomegranate during storage. The characterization of polyphenol oxidase in pomegranate fruit extract with respect to effect of enzyme concentration and substrate concentration was studied. For studying changes in polyphenol oxidase activity during storage, the pomegranate fruits at half yellow stage were freshly harvested and stored at ambient temperature and analyzed after 0, 2, 4, 6, 8 and 10 days of storage. The polyphenol oxidase activity has increased up to 8th day of storage and thereafter, decreased slightly on 10th day.

Keywords


Ambient, Half Yellow Stage, Substrate, Enzyme

References