Development and Validation of Bioanalytical Method for the Estimation of Carisoprodol in Human Plasma using LC-MS/MS

Simple, rapid and highly sensitive liquid chromatography- tandem mass spectrometric (LC-MS/MS) assay method was developed for the determination of Carisoprodol in human plasma. Carisoprodol methyl D3 was used as an internal standard (IS). The method employed 250μl of human plasma for sample processing by a simple Liquid Liquid Extraction (LLE) technique. The processed samples were chromatographer on a Phenyl column by using a mixture of 10mM Ammonium format - Acetonitrile (15:85,v/v) as the mobile phase at a flow rate of 1.2ml/min. The calibration curve obtained was linear over the concentration range of 25-3000ng/ml with r2 > 0.99. Method validation was performed as per the FDA guidelines and the results met the acceptance criteria. The Selected Ion Monitoring (SIM) mode was used for quantification of ion transitions at m/z 261.3/176.1 and 264.4/179.2 for the analyte and the IS respectively. A run time of 2.0 min was used which made it possible to analyze more than 400 plasma samples per day, thus increasing the productivity.