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Development and Validation of a RP-HPLC Method for Estimation of Thalidomide in Solid Dosage form
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A simple, rapid and precise isocratic reverse phase High Performance Liquid Chromatographic method has been developed for the determination of Thalidomide capsule. The separation was achieved with 150×4.6mm C18 column using mobile phase as Acetonitrile: Dimethyl Formamide : Water (60:10:30 v/v) at a flow rate of 1.0ml/min with UV detection at 297nm. The retention time of Thalidomide was found to be 2.17min. The linearity range of Thalidomide is 25mg to 200mg with coefficient of correlation 0.9986. The method is found to be accurate, precise and useful in quality control of dosage form.
Keywords
RP–HPLC, Thalidomide, Method Development and Validation
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