Asian Journal of Pharmaceutical Analysis

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Development and Validation of a RP-HPLC Method for Estimation of Thalidomide in Solid Dosage form


Affiliations
1 College of Pharmacy, Shree Ganpati Institute of Technology, NH-24, Opp. Jindal Pipes Ltd, Ghaziabad, (U.P.), Indonesia
2 College of Pharmacy, Shree Ganpati Institute of Technology, NH-24, Opp. Jindal Pipes Ltd, Ghaziabad, (U.P.), India
3 School of Pharmacy, Galghotia University, Greater Noida, G B Nagar, (U. P.), India
     

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A simple, rapid and precise isocratic reverse phase High Performance Liquid Chromatographic method has been developed for the determination of Thalidomide capsule. The separation was achieved with 150×4.6mm C18 column using mobile phase as Acetonitrile: Dimethyl Formamide : Water (60:10:30 v/v) at a flow rate of 1.0ml/min with UV detection at 297nm. The retention time of Thalidomide was found to be 2.17min. The linearity range of Thalidomide is 25mg to 200mg with coefficient of correlation 0.9986. The method is found to be accurate, precise and useful in quality control of dosage form.

Keywords

RP–HPLC, Thalidomide, Method Development and Validation
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  • Development and Validation of a RP-HPLC Method for Estimation of Thalidomide in Solid Dosage form

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Authors

Shiv Kumar Gupta
College of Pharmacy, Shree Ganpati Institute of Technology, NH-24, Opp. Jindal Pipes Ltd, Ghaziabad, (U.P.), Indonesia
Babita Kumar
College of Pharmacy, Shree Ganpati Institute of Technology, NH-24, Opp. Jindal Pipes Ltd, Ghaziabad, (U.P.), India
Pramod Kumar Sharma
School of Pharmacy, Galghotia University, Greater Noida, G B Nagar, (U. P.), India

Abstract


A simple, rapid and precise isocratic reverse phase High Performance Liquid Chromatographic method has been developed for the determination of Thalidomide capsule. The separation was achieved with 150×4.6mm C18 column using mobile phase as Acetonitrile: Dimethyl Formamide : Water (60:10:30 v/v) at a flow rate of 1.0ml/min with UV detection at 297nm. The retention time of Thalidomide was found to be 2.17min. The linearity range of Thalidomide is 25mg to 200mg with coefficient of correlation 0.9986. The method is found to be accurate, precise and useful in quality control of dosage form.

Keywords


RP–HPLC, Thalidomide, Method Development and Validation

References