Asian Journal of Pharmaceutical Analysis

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Development and Validation of UV Spectrophotometric Method for the Quantitative Estimation of Eugenol


Affiliations
1 Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, Hamdard Nagar, New Delhi – 110062,, India
2 Department of Pharmacognosy & Phytochemistry, Faculty of Pharmacy, Jamia Hamdard, Hamdard Nagar, New Delhi – 110062
3 Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, Hamdard Nagar, New Delhi – 110062, India
     

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Eugenol is an allyl chain-substituted guaiacol and finds variety of applications. Thus development of a validated UV spectrophotometric method will always be advantageous as the method is simple and rapid. The method was validated according to International Conference on Harmonisation (ICH) guidelines Q2(R1) with respect to linearity and range, precision, accuracy, detection limit (DL) and quantitation limit (QL). The detection limit (DL) and quantitation limit (QL) were determined as per the ICH guidelines and were found to be 0.82 and 2.48 μg mL-1 respectively. The method is expected to be useful in a variety of industries where eugenol finds its application.

Keywords

ICH, Linearity, Range, Precision, Accuracy, Detection Limit, Quantitation Limit
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  • Development and Validation of UV Spectrophotometric Method for the Quantitative Estimation of Eugenol

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Authors

K. Pramod
Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, Hamdard Nagar, New Delhi – 110062,, India
Shahid H. Ansari
Department of Pharmacognosy & Phytochemistry, Faculty of Pharmacy, Jamia Hamdard, Hamdard Nagar, New Delhi – 110062
Javed Ali
Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, Hamdard Nagar, New Delhi – 110062, India

Abstract


Eugenol is an allyl chain-substituted guaiacol and finds variety of applications. Thus development of a validated UV spectrophotometric method will always be advantageous as the method is simple and rapid. The method was validated according to International Conference on Harmonisation (ICH) guidelines Q2(R1) with respect to linearity and range, precision, accuracy, detection limit (DL) and quantitation limit (QL). The detection limit (DL) and quantitation limit (QL) were determined as per the ICH guidelines and were found to be 0.82 and 2.48 μg mL-1 respectively. The method is expected to be useful in a variety of industries where eugenol finds its application.

Keywords


ICH, Linearity, Range, Precision, Accuracy, Detection Limit, Quantitation Limit

References