RP-HPLC Determination of Atenolol in its Pharmaceutical Dosage Forms

A new and simple reverse phase HPLC method was developed for the estimation of atenolol in its pharmaceutical dosage forms. The mobile phase used acetonitrile, methanol, KH₂PO₄ is in the ratio of 250:250:500. Buffer solution was prepared by dissolving 0.05 M KH₂PO₄+0.1% H₃PO₄ adjunct with triethylamine. The separation was achieved on hypersil C-18 column, phenomenex Gemini(250×4.6 mm) and 5 μ particle size with rheodyne injector. The flow rate was 1 ml/min and uv detection at 238 nm. The retention time for atenolol was 4 min respectively. The linearity coefficient of atenolol was found to be 0.99% and the percentage recoveries for atenolol is 99.89%. The proposed method was accurate, precise and linear within the desired range. This method can successfully employed for the quantitative analysis of atenolol.