Asian Journal of Research in Chemistry

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Development and Validation of UV Spectrophotometric method for estimation of Ethamsylate in bulk and pharmaceutical dosage forms


Affiliations
1 A.R. College of Pharmacy and G.H. Patel Institute of Pharmacy, Vallabh Vidhyanagar, Dist: Anand, Gujarat
     

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A selective, simple, accurate and reproducible spectrophotometric method has been developed for the estimation of Ethamsylate in bulk and pharmaceutical formulation. The drug obeyed the Beer's law and showed good correlation. It showed absorption maxima at 300nm in 0.1N HCL. The developed method was validated with respect to linearity, accuracy and precision. The linearity was observed between 10-35μg/ml having line equationY=0.018x-0.0098with correlation coefficient of 0.999. Results of the analysis were validated statistically and by recovery study

Keywords

UV-Vis Spectrophotometer, Ethamsylate, Recovery, max
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  • British Pharmacopoeia, Her Majesty’s Stationery Office, London, Ph Eur Monograph 1204; 2005 vol-1, 762.

  • www.cemolink.com/msds/2624-44-4_130.doc.

  • http://en.wikipedia.org/wiki/Ethamsylate.

  • ONeil M.J, The Merk Index- an encyclopedia of chemicals, Drugs and Biologicals. 13th ed New Jersey, Merck and Co., 3757:664.

  • Sweetmann S C, Martindale – The Complete Drug Reference, 33rd ed London (UK), Pharmaceutical Press., 2002:732.

  • El-Enany Nahed., Belal Fathalla., Rizk Mohamed. Kinetic spectrophotometric determination of Ethamsylate in dosage forms. Journal of AOAC International 2007; 90(3):679-85.

  • Anju Goyal, Singhvi I. Spectrophotometric Estimation of simultaneous estimation of Etamsylate and Mefanamic acid from a Binary Mixture by Dual Wavelength and Simultaneous Equation Methods. Indian J. Pharm. Sci. 2008, 70(1), 105-108.

  • Roshan I., Kaushik KV., Diptish KN. Spectrophotometric methods for Simultaneous estimation of Ethamsylate and Tranexamic acid from combined tablet dosage form. Int J Chem Tech Res. 2010, 2(1), 74-8.

  • Roshan I., Kaushik KV., Diptish KN. Spectrophotometric methods for Simultaneous estimation of Ethamsylate and Tranexamic acid from combined tablet dosage form. Int J Chem Tech Res. 2010, 2(1),74-8.

  • Yogini SJ., Gokl ST., Sanjay JS. A simple and sensitive HPTLC method for quantitative analysis of Ethamsylate in tablets. J Planar Chrom. Sep/Oct; 2005, 18,380-3.

  • International conference on Harmonization, Guidance for Industry In; Q2B validation of analytical procedures, Methodology, Switzerland; IFPMA 1996;1-8

  • International conference on Harmonization, Guidance for Industry In; Q2A Text on validation of analytical methods, Switzerland; IFPMA 1994;1-4.


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  • Development and Validation of UV Spectrophotometric method for estimation of Ethamsylate in bulk and pharmaceutical dosage forms

Abstract Views: 422  |  PDF Views: 2

Authors

Priti. H. Patel
A.R. College of Pharmacy and G.H. Patel Institute of Pharmacy, Vallabh Vidhyanagar, Dist: Anand, Gujarat
Vaishali V. Kharkhanis
A.R. College of Pharmacy and G.H. Patel Institute of Pharmacy, Vallabh Vidhyanagar, Dist: Anand, Gujarat

Abstract


A selective, simple, accurate and reproducible spectrophotometric method has been developed for the estimation of Ethamsylate in bulk and pharmaceutical formulation. The drug obeyed the Beer's law and showed good correlation. It showed absorption maxima at 300nm in 0.1N HCL. The developed method was validated with respect to linearity, accuracy and precision. The linearity was observed between 10-35μg/ml having line equationY=0.018x-0.0098with correlation coefficient of 0.999. Results of the analysis were validated statistically and by recovery study

Keywords


UV-Vis Spectrophotometer, Ethamsylate, Recovery, max

References