Asian Journal of Research in Chemistry

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Development and Validation by RP-HPLC Method for Estimation of Zidovudine in Bulk and Its Pharmaceutical Dosage Form


Affiliations
1 Ganga Pharmacy College, Nizamabad, A.P, India
2 University College of Pharmaceutical Sciences, Palamur University, Mahabubnagar
     

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A rapid and precise reverse phase high performance liquid chromatographic method has been developed for the validated of zidovudine, in its pure form as well as in tablet dosage form. Chromatography was carried out on a symmetry C18 (4.6 x 150mm, 5µm) column using a mixture of, methanol and phosphate buffer (65:35 v/v) as the mobile phase at a flow rate of 1.0ml/min, the detection was carried out at 264nm. The retention time of the drug was 2.55±0.02min.The method produce linear responses in the concentration range of 20-80mg/ml of zidovudine. The method precision for the determination of assay was below 2.0%RSD. The method is useful in the quality control of bulk and pharmaceutical formulations

Keywords

Zidovudine, RP-HPLC, Validation
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  • Development and Validation by RP-HPLC Method for Estimation of Zidovudine in Bulk and Its Pharmaceutical Dosage Form

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Authors

M.A. Haneef
Ganga Pharmacy College, Nizamabad, A.P, India
B. Rajkamal
Ganga Pharmacy College, Nizamabad, A.P, India
G. Tharun goud
University College of Pharmaceutical Sciences, Palamur University, Mahabubnagar
V. Mohan Goud
University College of Pharmaceutical Sciences, Palamur University, Mahabubnagar

Abstract


A rapid and precise reverse phase high performance liquid chromatographic method has been developed for the validated of zidovudine, in its pure form as well as in tablet dosage form. Chromatography was carried out on a symmetry C18 (4.6 x 150mm, 5µm) column using a mixture of, methanol and phosphate buffer (65:35 v/v) as the mobile phase at a flow rate of 1.0ml/min, the detection was carried out at 264nm. The retention time of the drug was 2.55±0.02min.The method produce linear responses in the concentration range of 20-80mg/ml of zidovudine. The method precision for the determination of assay was below 2.0%RSD. The method is useful in the quality control of bulk and pharmaceutical formulations

Keywords


Zidovudine, RP-HPLC, Validation

References