Asian Journal of Research in Chemistry

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Development and Validation by RP-HPLC Method for Estimation of Zidovudine in Bulk and Its Pharmaceutical Dosage Form


Affiliations
1 Ganga Pharmacy College, Nizamabad, A.P, India
2 University College of Pharmaceutical Sciences, Palamur University, Mahabubnagar
     

   Subscribe/Renew Journal


A rapid and precise reverse phase high performance liquid chromatographic method has been developed for the validated of zidovudine, in its pure form as well as in tablet dosage form. Chromatography was carried out on a symmetry C18 (4.6 x 150mm, 5µm) column using a mixture of, methanol and phosphate buffer (65:35 v/v) as the mobile phase at a flow rate of 1.0ml/min, the detection was carried out at 264nm. The retention time of the drug was 2.55±0.02min.The method produce linear responses in the concentration range of 20-80mg/ml of zidovudine. The method precision for the determination of assay was below 2.0%RSD. The method is useful in the quality control of bulk and pharmaceutical formulations

Keywords

Zidovudine, RP-HPLC, Validation
Subscription Login to verify subscription
User
Notifications
Font Size


  • Anonymous 1.URL: http://en.wikipedia.org/wiki/Zidovudine

  • Anonymous 2.Indian pharmacopoeia-2007, volume-3.

  • Meyer VR. Practical high performance liquid chromatography. 2nd ed. London: John Wiley and Sons; 1993.

  • Kazakevich Y, Lobrutto R.HPLC for pharmaceutical scientists. London: John Wiley and Sons; 2007.

  • ICH: Q2B, Analytical Validation- Methodology, 1996.

  • David M, Bliesner. Validating Chromatographic Methods - A Practical Guide - New Jersey: John Wiley and Sons Inc.; 2006.p. 1, 9-12.

  • Paul C Sadek. The HPLC Solvent Guide. 2nd Ed. New York: Wiley-Interscience John Wiley and Sons Inc.; 2007. p. 67-74

  • Ermer J, Miller JH Mc; B. Method Validation in Pharmaceutical Analysis-A Guide to Best Practice. Weinheim: Wiley-Vch Verlag GmbH and Co. KgaA; 2005. p. 21, 52, 63, 80, 99, 101, 120, 170.

  • Hokanson GC. A life cycle approach to the validation of analytical methods during pharmaceutical product development.

  • Riley CM, Thomas WR. Development and validation of analytical methods. Vol.III. United states of America: Elsevier Sciences Inc; 1996.


Abstract Views: 368

PDF Views: 2




  • Development and Validation by RP-HPLC Method for Estimation of Zidovudine in Bulk and Its Pharmaceutical Dosage Form

Abstract Views: 368  |  PDF Views: 2

Authors

M.A. Haneef
Ganga Pharmacy College, Nizamabad, A.P, India
B. Rajkamal
Ganga Pharmacy College, Nizamabad, A.P, India
G. Tharun goud
University College of Pharmaceutical Sciences, Palamur University, Mahabubnagar
V. Mohan Goud
University College of Pharmaceutical Sciences, Palamur University, Mahabubnagar

Abstract


A rapid and precise reverse phase high performance liquid chromatographic method has been developed for the validated of zidovudine, in its pure form as well as in tablet dosage form. Chromatography was carried out on a symmetry C18 (4.6 x 150mm, 5µm) column using a mixture of, methanol and phosphate buffer (65:35 v/v) as the mobile phase at a flow rate of 1.0ml/min, the detection was carried out at 264nm. The retention time of the drug was 2.55±0.02min.The method produce linear responses in the concentration range of 20-80mg/ml of zidovudine. The method precision for the determination of assay was below 2.0%RSD. The method is useful in the quality control of bulk and pharmaceutical formulations

Keywords


Zidovudine, RP-HPLC, Validation

References