Asian Journal of Research in Chemistry

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Analytical Method Development and Validation for Simultaneous Determination of Ambroxol and Olopatadine Hydrochloride in Synthetic Mixture


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1 Parul Institute of Pharmacy, Waghodia, Limda, Gujarat, India
     

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An Absoption Correction UV spectrophotometric and a reversed Phase high-performance liquid chromatographic method for the determination of Ambroxol and Olopatadine Hydrochloride in Synthetic mixture were developed. The Absorption Correction UV spectrophotometric method was found to be accurate with 99.75±0.04 recovery for ambroxol and recovery for olopatadine 99.89±0.02 respectively and method is also precise with a coefficient of variation for olopatadine and ambroxol was 0.996 and 0.885 respectively. For reverse phase HPLC, A reversed-phase C18 column with methanol: ammonium acetate buffer (80:20, v/v, pH 5.5) mobile phase was used and the detector wavelength was set at 244 nm. Linearity range for ambroxol and Olopatadine Hcl was found to be 78-150 μg/ml and 13-25 µg/ml respectively.

Keywords

Ambroxol, Olopatadine Hydrochloride, Absoption Correction UV Spectrophotometry, Reversed Phase HPLC
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  • Analytical Method Development and Validation for Simultaneous Determination of Ambroxol and Olopatadine Hydrochloride in Synthetic Mixture

Abstract Views: 986  |  PDF Views: 3

Authors

Ujjaval N. Limbachiya
Parul Institute of Pharmacy, Waghodia, Limda, Gujarat, India
Nikita N. Patel
Parul Institute of Pharmacy, Waghodia, Limda, Gujarat, India
Rana Nikesh
Parul Institute of Pharmacy, Waghodia, Limda, Gujarat, India
K.S Rajesh
Parul Institute of Pharmacy, Waghodia, Limda, Gujarat, India
Shreya Patel
Parul Institute of Pharmacy, Waghodia, Limda, Gujarat, India
Gajanan Shinde
Parul Institute of Pharmacy, Waghodia, Limda, Gujarat, India

Abstract


An Absoption Correction UV spectrophotometric and a reversed Phase high-performance liquid chromatographic method for the determination of Ambroxol and Olopatadine Hydrochloride in Synthetic mixture were developed. The Absorption Correction UV spectrophotometric method was found to be accurate with 99.75±0.04 recovery for ambroxol and recovery for olopatadine 99.89±0.02 respectively and method is also precise with a coefficient of variation for olopatadine and ambroxol was 0.996 and 0.885 respectively. For reverse phase HPLC, A reversed-phase C18 column with methanol: ammonium acetate buffer (80:20, v/v, pH 5.5) mobile phase was used and the detector wavelength was set at 244 nm. Linearity range for ambroxol and Olopatadine Hcl was found to be 78-150 μg/ml and 13-25 µg/ml respectively.

Keywords


Ambroxol, Olopatadine Hydrochloride, Absoption Correction UV Spectrophotometry, Reversed Phase HPLC

References