Asian Journal of Research in Chemistry

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Improved Production of L-Lysine by Immobilized Biotin Auxotropic Mutant Micrococcus glutamicus AB200


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1 Department of Chemical Engineering, University of Calcutta, Kolkata – 700 009
     

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Experimental studies were carried out to examine the efficiency of whole cell immobilization in calcium alginate beads and agar blocks for the production of L-lysine using a biotin autotrophic mutant Micrococcus glutamicus AB<SUB>200</SUB> developed in laboratory by induced mutation from a regulatory mutant Micrococcus glutamicus AB5. Under the same physico- chemical environment, using selected suitable synthetic medium, different parameters for both calcium alginate beads and agar blocks were optimized one by one to maximize the production of L-lysine. Production was increased significantly with cells entrapped in calcium alginate beads compared to free cells with the presence of 0.2(M) CaCl<SUB>2</SUB> in synthetic medium, 0.2 (M) CaCl<SUB>2</SUB> for bead formation, 4% sodium alginate, 24 h storage period of beads and 1.5 cell/alginate ratio but the accumulation of was decreased significantly with the whole cell entrapped in agar blocks, compared to free cells.

Keywords

Immobilization, L-lysine, Calcium Alginate Bead, Encapsulation, Mutant
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  • Improved Production of L-Lysine by Immobilized Biotin Auxotropic Mutant Micrococcus glutamicus AB200

Abstract Views: 365  |  PDF Views: 1

Authors

A. Gupta
Department of Chemical Engineering, University of Calcutta, Kolkata – 700 009
S. K. Das
Department of Chemical Engineering, University of Calcutta, Kolkata – 700 009
A. K. Banik
Department of Chemical Engineering, University of Calcutta, Kolkata – 700 009

Abstract


Experimental studies were carried out to examine the efficiency of whole cell immobilization in calcium alginate beads and agar blocks for the production of L-lysine using a biotin autotrophic mutant Micrococcus glutamicus AB<SUB>200</SUB> developed in laboratory by induced mutation from a regulatory mutant Micrococcus glutamicus AB5. Under the same physico- chemical environment, using selected suitable synthetic medium, different parameters for both calcium alginate beads and agar blocks were optimized one by one to maximize the production of L-lysine. Production was increased significantly with cells entrapped in calcium alginate beads compared to free cells with the presence of 0.2(M) CaCl<SUB>2</SUB> in synthetic medium, 0.2 (M) CaCl<SUB>2</SUB> for bead formation, 4% sodium alginate, 24 h storage period of beads and 1.5 cell/alginate ratio but the accumulation of was decreased significantly with the whole cell entrapped in agar blocks, compared to free cells.

Keywords


Immobilization, L-lysine, Calcium Alginate Bead, Encapsulation, Mutant

References