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Spectrophotometric Determination of Oxcarbazepine in Bulk and Pharmaceutical Formulations
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Two new rapid, simple, sensitive reproducible and economical spectrophotometric methods are described for the determination of Oxcarbazepine (OXC) in bulk form. Both methods are based on the formation of colored complexes due to the action of 4-Amino phenazone and IsoNiconitic Hydrazide on OXC in alcoholic medium. Under optimized conditions, they show an absorption maxima at 440nm (4-AP) and 450nm (INH), with molar absorptivities of 3.824 x10<SUP>4</SUP> and 2.838x10<SUP>4</SUP> mole- cm- and sand ells sensitivities of 0.08474 and 0.13968 per 0.001 absorbance unit for 4-AP and INH, respectively. The color is stable for 5 min after extraction. In both cases Beers law is obeyed between 2.5- 12.5 µg/ ml. The proposed method was successfully extended to bulk and Pharmaceutical dosage forms.
Keywords
Oxcarbazepine (OXC) 4-amino phenazone (4-AP), Isonicotinic hydrazide (INH), Spectrophotometry, Condensation
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