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Spectrophotometric Determination of Oxcarbazepine in Bulk and Pharmaceutical Formulations
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Two new rapid, simple, sensitive reproducible and economical spectrophotometric methods are described for the determination of Oxcarbazepine (OXC) in bulk form. Both methods are based on the formation of colored complexes due to the action of 4-Amino phenazone and IsoNiconitic Hydrazide on OXC in alcoholic medium. Under optimized conditions, they show an absorption maxima at 440nm (4-AP) and 450nm (INH), with molar absorptivities of 3.824 x10<SUP>4</SUP> and 2.838x10<SUP>4</SUP> mole- cm- and sand ells sensitivities of 0.08474 and 0.13968 per 0.001 absorbance unit for 4-AP and INH, respectively. The color is stable for 5 min after extraction. In both cases Beers law is obeyed between 2.5- 12.5 µg/ ml. The proposed method was successfully extended to bulk and Pharmaceutical dosage forms.
Keywords
Oxcarbazepine (OXC) 4-amino phenazone (4-AP), Isonicotinic hydrazide (INH), Spectrophotometry, Condensation
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- Murray, L., In; Physician's Desk Reference, 56th Edn., Thomson Health care, 2002, 2407.
- Grant, S. M. and Faulds, D., Drugs, 1992, 43, 873.
- Fraser, A. D., Clin. Biochem., 1996, 29, 97.
- Kalis, M. M. and Huff, N. A., Clin. Ther., 2001, 23, 680.
- Qi, M. L., Wang, P., Wang, L. J. and Fu, R. N., J. Pharm. Biomed. Anal., 2003, 31, 57.
- Rouan, M. C., Decherf, M., Le Clanche, V., Lecaillon, J. B. and Godbillon, J., J. Chromatogr. B Biomed. Appl., 1994, 658, 167
- Matar, K. M., Nicholls- al-Hassan, M. I. and Tekle, A., J. Clin. Pharm. Ther., 1995, 20, 229
- Rouan MC, Decherf M, Le Clanche V, Lecaillon JB, Godbillon J. Automated microanalysis of oxcarbazepine and its monohydroxy and transdiol metabolites in plasma by liquid chromatography. J Chromatogr B Biomed Appl.1994; 658(1): 167-72.
- Mandrioli R, Ghedini N, Albani F, Kenndler E , Raggi MA. Liquid chromatographic determination of oxcarbazepine and its metabolites in plasma of epileptic patients after solid-phase extraction. J Chromatogr B: Analyt Technol Biomed Life Sci. 2003; 783(1): 253-63.
- Manuela Contin, Monica Balboni, Erica Callegati, Carmina Candela, Fiorenzo Albani, Roberto Riva , Agostino Baruzzi. Simultaneous liquid chromatographic determination of lamotrigine, oxcarbazepine monohydroxy derivative and felbamate in plasma of patients with epilepsy. J Chromatogr B: Analyt Technol Biomed Life Sci. 2005; 828(1-2): 113-7.
- Levert H, Odou P, Robert H. LC determination of oxcarbazepine and its active metabolite in human serum. J Pharma Biomed Anal. 2002; 28(3-4): 517-25.
- VanBelle K, Dekoster V, Sarre S, Ebinger G , Michotte Y. Liquid chromatographic assay using a microcolumn coupled to a Ushaped optical cell for high-sensitivity ultraviolet absorbance detection of oxcarbazepine and its major metabolite in microdialysates. J Chromatogr B: Biomed Appl.1994; 657(1): 149-54.
- Menge GP, Dubois JP, Bauer G. Simultaneous determination of carbamazepine, oxcarbazepine and their main metabolites in plasma by liquid chromatography. J Chromatogr.1987; 414(2): 477- 83.
- Qi ML, Wang P, Wang LJ, Fu RN. LC method for the determination of oxcarbazepine in pharmaceutical preparations. J Pharma Biomed Anal. 2003; 31: 57-62.
- Ramaa CS, Chothe PP, Naik AA, Kadam VJ. Spectrophotometric method for the estimation of oxcarbazepine in tablets. Indian Journal of Pharmaceutical Sciences.2006; 68(2): 265-266.
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