Asian Journal of Research in Chemistry

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Development and Validation of Simultaneous Equation UV-Spectrophotometric Method for the Estimation of Metformin HCl and Glimepiride in Combined Dosage Form


Affiliations
1 Department of Pharmaceutical Chemistry, IIMT College of Medical Sciences, Meerut-250001
2 HOD, Department of Pharmaceutical Chemistry, IIMT College of Medical Sciences, Meerut
     

   Subscribe/Renew Journal


A simple, sensitive, precise and specific UV spectrophotometric method for simultaneous estimation of Metformin HCl and Glimepiride in combined dosage form has been developed. Two wavelengths 233 nm and 228.4 nm were selected for estimation of Metformin HCl and Glimepiride respectively. Acetonitrile was used as solvent for analysis of both the drugs. Linearity was observed in the concentration range of 5-10 μg/ml (r2=0.9992) and 3-18 μg/ml (r2=0.9999) for Metformin HCl and Glimepiride respectively. The method was validated as per ICH guidelines.
Subscription Login to verify subscription
User
Notifications
Font Size


  • Klepser T.B., Kelly M.W.; Metformin hydrochloride: An antihyperglycemic agent. Am J Health System Pharm. 1997; 54:893–903.

  • Hitt E.;Uses of metformin may extend beyond patients with type 2 diabetes. Drugs. 1995; 63:1879–94.

  • Maria C.R., Ranetti A.;The pharmacokinetics of some oral antidiabetics –A pharmaceutical approach. Farmacia, 2006; LIV, 6, 3- 13.

  • Hermann L. S.; Metformin:A review of its pharmacological properties and therapeutic use. Diabete metab. 1979; 5(3): 233- 45.

  • Budavari S, in. The Merck Index., ed. Merck and Co Inc., 13thed., 1997; Whitehouse Station (NJ), 5792.

  • Arayne MS, Sultana N, Zuberi MH, Siddiqui FA. Spectrophotometric quantitation of Metformin in bulk drug and pharmaceutical formulations using multivariate technique. Indian J Pharm Sci 2009; 71 (3): 331-335.

  • Chaturvedi PK, Sharma R. Simultaneous spectrophotometric estimation and validation of three component tablet formulation containing pioglitazone hydrochloride, Metformin hydrochloride and Glibenclamide. Analytical Letters 2008; 41(12):2133-2142.

  • Rohokale BS, Jadhav VM, Kadam VJ. Studies in prospective process validation of Metformin HCl tablet Dosage formulation. Int J PharmaTech Res 2010; 2(3):1673-1678.

  • Satoskar RG, Bhandarkar SD, Nirmala N, pharmacology and pharmacotherapeutics, Vol II,17th ed, Popular publication, Mumbai, Maharastra, India.

  • Muller G, Hartz D, Punter J, Okonomopulos R, Kramer W; Differential interaction of glimepiride and glibenclamide with the beta-cell sulfonylurea receptor. I. Binding characteristics. Biochim Biophys Acta. 1994; 1191(2):267-77.

  • ICH, Q2 (R1) Validation of analytical procedure: Text and methodology, International Conference on Harmonization, Geneva, Switzerland, 2005.


Abstract Views: 415

PDF Views: 2




  • Development and Validation of Simultaneous Equation UV-Spectrophotometric Method for the Estimation of Metformin HCl and Glimepiride in Combined Dosage Form

Abstract Views: 415  |  PDF Views: 2

Authors

Atul Srivastav
Department of Pharmaceutical Chemistry, IIMT College of Medical Sciences, Meerut-250001
Preeti Tiwari
HOD, Department of Pharmaceutical Chemistry, IIMT College of Medical Sciences, Meerut
Shishir Maheshwari
Department of Pharmaceutical Chemistry, IIMT College of Medical Sciences, Meerut-250001

Abstract


A simple, sensitive, precise and specific UV spectrophotometric method for simultaneous estimation of Metformin HCl and Glimepiride in combined dosage form has been developed. Two wavelengths 233 nm and 228.4 nm were selected for estimation of Metformin HCl and Glimepiride respectively. Acetonitrile was used as solvent for analysis of both the drugs. Linearity was observed in the concentration range of 5-10 μg/ml (r2=0.9992) and 3-18 μg/ml (r2=0.9999) for Metformin HCl and Glimepiride respectively. The method was validated as per ICH guidelines.

References