Asian Journal of Research in Chemistry

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Development and Validation of Simultaneous Equation UV-Spectrophotometric Method for the Estimation of Metformin HCl and Glimepiride in Combined Dosage Form


Affiliations
1 Department of Pharmaceutical Chemistry, IIMT College of Medical Sciences, Meerut-250001
2 HOD, Department of Pharmaceutical Chemistry, IIMT College of Medical Sciences, Meerut
     

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A simple, sensitive, precise and specific UV spectrophotometric method for simultaneous estimation of Metformin HCl and Glimepiride in combined dosage form has been developed. Two wavelengths 233 nm and 228.4 nm were selected for estimation of Metformin HCl and Glimepiride respectively. Acetonitrile was used as solvent for analysis of both the drugs. Linearity was observed in the concentration range of 5-10 μg/ml (r2=0.9992) and 3-18 μg/ml (r2=0.9999) for Metformin HCl and Glimepiride respectively. The method was validated as per ICH guidelines.
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  • Development and Validation of Simultaneous Equation UV-Spectrophotometric Method for the Estimation of Metformin HCl and Glimepiride in Combined Dosage Form

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Authors

Atul Srivastav
Department of Pharmaceutical Chemistry, IIMT College of Medical Sciences, Meerut-250001
Preeti Tiwari
HOD, Department of Pharmaceutical Chemistry, IIMT College of Medical Sciences, Meerut
Shishir Maheshwari
Department of Pharmaceutical Chemistry, IIMT College of Medical Sciences, Meerut-250001

Abstract


A simple, sensitive, precise and specific UV spectrophotometric method for simultaneous estimation of Metformin HCl and Glimepiride in combined dosage form has been developed. Two wavelengths 233 nm and 228.4 nm were selected for estimation of Metformin HCl and Glimepiride respectively. Acetonitrile was used as solvent for analysis of both the drugs. Linearity was observed in the concentration range of 5-10 μg/ml (r2=0.9992) and 3-18 μg/ml (r2=0.9999) for Metformin HCl and Glimepiride respectively. The method was validated as per ICH guidelines.

References