Asian Science

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

In Vitro Anticancer and Hepatoprotective Activity Studies of Garcinia xanthochymus


Affiliations
1 Dr. P. Sadananda Maiya Centre for Food Science and Research, Jayanagara, Bengaluru (Karnataka), India
2 Department of Studies and Research in Chemistry, Tumkur University, Tumkur (Karnataka), India
     

   Subscribe/Renew Journal


Natural products are very well known to exhibit anticancer activities. The present study aimed at assessing the potency of various extracts of Garcinia xanthochymus against cancer using in vitro cell lines. MCF 7, HepG2, HeLa, PC3, A549 and Vero cell lines were employed for the assessment. The ability of extracts to exert toxic insult on cancer cells has been the basis of anticancer activity. GxF was fond to be very potentially toxic (80 mg/ml) to HEP G2 cell lines among all the tested extracts. Also, it was found to be the most toxic compared to GxA and GxF whose average CTC50 was found to be 180 and 118 mg/ml, respectively. Among all the tested extracts, GxF was found to be potentially toxic to the MCF 7 cell lines whose CTC50 was found to be73 mg/ml. GxA possesses the CTC50 of 810 mg/ ml. GxR was found to be toxic with average CTC50 of 303 mg/ml. This was followed by GxF and GxA with average CTC50 values of 303 and 456 mg/ml. In vitro hepatoprotective activity of the plant extracts was studied by employing primary rat hepatocytes. The drug silymarin was found to exhibit 85.28 per cent protection against paracetamol induced toxicity in primary rat hepatocytes at the tested concentration of 250 mg/ml. It was found that GxF and GxR were found to have comparatively similar protective power like silymarin. These extract exhibited 83.63 per cent and 79.58 per cent protection against paracetamol induced toxicity in primary rat hepatocytes at the concentration of 200 mg/ml, respectively. GxA did not exhibit considerable activity with 55.61 per cent protection.

Keywords

Garcinia xanthochymus, In Vitro, Anticancer, Hepatoprotective, Cell Lines.
Subscription Login to verify subscription
User
Notifications
Font Size


  • Ambasta, S.P. (1986). The useful plants of India; Publications and Information Directorate, Council of Scientific and Industrial Research: New Delhi, 231pp.

  • Baggett, S., Protiva, P., Mazzola, E.P., Yang, H., Ressler, E.T., Basile, M.J., Weinstein, I.B. and Kennelly, E.J. (2005). Bioactive benzophenones from Garcinia xanthochymus fruits. J. Nat. Prod., 68(3): 354-360.

  • Baslas, R.K. and Kumar, P. (1981). Isolation and characterisation of biflavanone and xanthones in the fruits of Garcinia xanthochymus. Acta Ciencia Indica Chem., 7(1): 31-34.

  • Bawa, A.S. and Khanum, F. (2003). Nutraceuticals: Mechanism of action. Indian Food Industry, 22(6): 44-51.

  • Bennett, G.J. and Lee, H.H. (1989). Xanthones from Guttiferae. Phytochemistry, 28(4): 967-998.

  • Canter, P.H., Thomas, H. and Ernst, E. (2005). Bringing medicinal plants into cultivation: opportunities and challenges for biotechnology. Trends in Biotechnol., 23: 180-185.

  • Chandalia, M., Garg, A., Lutjohnn, D., Von Bergmann, K., Grundy, S.M. and Brinkley, L.J. (2000). Beneficial effects of high dietary fibre intake in patients with type II diabetes mellitus. New England J. Med., 342: 1392-1398.

  • Chanmahasathien, W., Li, Y., Satake, M., Oshima, Y., Ishibashi, M., Ruangrungsi, N. and Ohizumi, Y. (2003a). Prenylated xanthones fromGarcinia xanthochymus. Chem. Pharm. Bull. Tokyo, 51(11) : 1332-1334.

  • Chanmahasathien, W., Li, Y., Satake, M., Oshima, Y., Ruangrungsi, N. and Ohizumi, Y. (2003b). Prenylated xanthones with NGF-Potentiating activity from Garcinia xanthochymus. Phytochemistry, 64(5): 981-986.

  • Chen, Y., Fan, H., Yang,G.Z., Jiang, Y., Zhong, F.F. and He, H.W. (2010). Prenylated xanthones from the bark of Garcinia xanthochymus and their 1, 1'-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities. Molecules, 15(10): 7438-7449.

  • Das, P., Goswami, K., Chinniah, S., Panda, A., Banerjee, N., Sahu, S. and Achari, N.P.B. (2007). Wood for dia fruticosa: traditional uses and recent findings. J. Ethnopharmacol., 110: 189-199.

  • Duthie, G.G. and Brown, K.M. (1994). Reducing the risk of cardiovascular disease, published in functional foods, designer foods, pharma foods and nutraceutical. Chapman and Hall Publishers, New York, USA (Ed.) Israel Goldberg. 78-85pp.

  • Facciola, S. (1998). Cornucopia II: A source book of edible plants; Kampong Publications: California, 79pp.

  • Faisal, Z.A.M., Sotheeswaran, S. and Wijesundera, C. (1982). Economically useful plants of Sri Lanka. Part V. Seed fats of some Garcinia species (Guttiferae), ibid. J. Natl. Sci. Counc. Sri Lanka. 10: 221-223.

  • Favero, J., Corbeau, P., Nicolas, M., Benkirane, M., Trave, G., Dixon, J.F.P., Aucouturier, P., Rasheed, S., Liautard, J.P.,

  • Devaux, C. and Dornand, J. (1993). Inhibition of human immuno deficiency virus infection by the lectin Jacalin and by a derived peptide showing a sequence similarity with gp120. European J. Immunol., 23: 179-185.

  • Gustafson, K.R., Blunt, J.W., Munro, M.H.G., Fuller, R.W.,

  • McKee, T.C., Cardellina, J.H., McMahon, J.B., Cragg, G.M. and Boyd, M.R. (1992). The guttiferones, HIV-inhibitory benzophenones fromSymphonia globulifera, Garcinia livingstonei, Garcinia ovalifolia and Clusia rosea. Tetrahedron, 48: 10093-10102.

  • Gutierrez, R.M.P., Mitchell, S. and Solis, R.V. (2008). Psidium guajava: a review of its traditional uses, phytochemistry and pharmacology. J. Ethnopharmacol., 117: 1-27.


Abstract Views: 501

PDF Views: 0




  • In Vitro Anticancer and Hepatoprotective Activity Studies of Garcinia xanthochymus

Abstract Views: 501  |  PDF Views: 0

Authors

S. J. Prashanth
Dr. P. Sadananda Maiya Centre for Food Science and Research, Jayanagara, Bengaluru (Karnataka), India
D. Suresh
Department of Studies and Research in Chemistry, Tumkur University, Tumkur (Karnataka), India
V. H. Potty
Dr. P. Sadananda Maiya Centre for Food Science and Research, Jayanagara, Bengaluru (Karnataka), India
P. Sadananda Maiya
Dr. P. Sadananda Maiya Centre for Food Science and Research, Jayanagara, Bengaluru (Karnataka), India

Abstract


Natural products are very well known to exhibit anticancer activities. The present study aimed at assessing the potency of various extracts of Garcinia xanthochymus against cancer using in vitro cell lines. MCF 7, HepG2, HeLa, PC3, A549 and Vero cell lines were employed for the assessment. The ability of extracts to exert toxic insult on cancer cells has been the basis of anticancer activity. GxF was fond to be very potentially toxic (80 mg/ml) to HEP G2 cell lines among all the tested extracts. Also, it was found to be the most toxic compared to GxA and GxF whose average CTC50 was found to be 180 and 118 mg/ml, respectively. Among all the tested extracts, GxF was found to be potentially toxic to the MCF 7 cell lines whose CTC50 was found to be73 mg/ml. GxA possesses the CTC50 of 810 mg/ ml. GxR was found to be toxic with average CTC50 of 303 mg/ml. This was followed by GxF and GxA with average CTC50 values of 303 and 456 mg/ml. In vitro hepatoprotective activity of the plant extracts was studied by employing primary rat hepatocytes. The drug silymarin was found to exhibit 85.28 per cent protection against paracetamol induced toxicity in primary rat hepatocytes at the tested concentration of 250 mg/ml. It was found that GxF and GxR were found to have comparatively similar protective power like silymarin. These extract exhibited 83.63 per cent and 79.58 per cent protection against paracetamol induced toxicity in primary rat hepatocytes at the concentration of 200 mg/ml, respectively. GxA did not exhibit considerable activity with 55.61 per cent protection.

Keywords


Garcinia xanthochymus, In Vitro, Anticancer, Hepatoprotective, Cell Lines.

References