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Ameliorative Effects of the Homeopathic Medicine Lycopodium 200c and Extract of Phyllanthus emblica in Cadmium-Induced Neurotoxicity in Mice


Affiliations
1 Genetics and Molecular Biology Unit, Department of Zoology, Vidyasagar University, Midnapore 721 102, India
2 Department of Zoology, Vidyasagar College, Kolkata 700 006, India
 

Cadmium is an extremely toxic heavy metal and causes neurotoxicity by inducing oxidative stress and membrane disturbances in brain. Phyllanthus emblica and Lycopodium 200c have anti-oxidative properties and are able to remove the cadmium-produced free radicals. This study investigates the role of Lycopodium 200c and Phyllanthus emblica (amlaki) in ameliorating the toxic effects of cadmium on the brain of mice. Swiss albino mice were used and divided into four different sets with one control, one induced, one with amlaki and other with both amlaki and Lycopodium treatment. To observe the changes, tests for brain acetylcholinesterase along with Mg2+ ATPase activities were performed. Results show that cadmium toxicity leads to decrease in enzymatic activities which can be reversed by the effects of amlaki and Lycopodium 200c.

Keywords

Antioxidative Properties, Cadmium, Free Radicals, Oxidative Stress, Toxicity.
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  • Webster, W. S. and Valois, A. A., The toxic effect of cadmium on the neonatal mouse CNS. J. Neuropathol. Exp. Neurol., 1981, 40, 247–257.

  • Ahammadsahib, K. I., Jinna, R. R. and Desaiah, D., Protection against cadmium toxicity and enzyme inhibition by dithiothreitol. Cell Biochem. Funct., 1989, 7(3), 185–192.

  • Stohs, S. J. and Bagchi, D., Oxidative mechanisms in the toxicity of metal ions. Free Radical Biol. Med., 1995, 18, 321–336.

  • Galaris, D. and Evangelou, A., The role of oxidative stress in mechanisms of metal-induced carcinogenesis. Crit. Rev. Oncol. Hematol., 2002, 42, 93–103.

  • Hobson, M. V., Milhouse, M. and Rajanna, B., Effects of cadmium on the uptake of dopamine and norepinephrine in rat brain synaptosomes. Bull. Environ. Contam. Toxicol., 1986, 37, 421–426.

  • Carageorgiou, H., Tzotzes, V., Sideris, A., Zarros, A. and Tsakiris, S., Cadmium effects on brain acetylcholinesterase activity and antioxidant status of adult rats: modulation by zinc, calcium and L-cysteine co-administration. Basic Clin. Pharmacol. Toxicol., 2005, 97, 320–324.

  • Cooper, G. P., Suszkiw, J. B. and Manalis, R. S., Heavy metals: effects on synaptic transmission. Neurotoxicology, 1984, 5(3), 247–266.

  • Kouniniotou-Krontiri, P. and Tsakiris, S., Time dependence of Li+ action on acetylcholinesterase activity in correlation with spontaneous quantal release of acetylcholine in rat diaphragm. Jap. J. Physiol., 1989, 39, 429–440.

  • Klegeris, A., Korkina, L. G. and Greenfield, S. A., A possible interaction between acetylcholinesterase and dopamine molecules during autoxidation of the amine. Free Radic. Bio. Med., 1995, 18, 223–230.

  • Wang, S. H., Shih, Y. L., Ko, W. C., Wei, Y. H. and Shih, C. M., Cadmium-induced autophagy and apoptosis are mediated by a calcium signaling pathway. Cell Mol. Life Sci., 2008, 65, 3640–3652.

  • Renugadevi, J. and Milton, Prabu, S., Naringenin protects against cadmium induced oxidative renal dysfunction in rats. Toxicology, 2009, 256, 128–134.

  • Misra, U. K., Gawdi, G., Akabani, G. and Pizzo, S. V., Cadmiuminduced DNA synthesis and cell proliferation in macrophages: the role of intracellular calcium and signal transduction mechanisms. Cell. Signal, 2002, 14, 327–340.

  • Ellman, G. L., Courtney, K. D., Andres Jr, V. and Feather-Stone, R. M., A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem. Pharmacol., 1961, 7, 88–95.

  • Ohinishi, T., Suzuki, T., Suzuki, Y. and Ozawa, K., Comparative study of plasma membrane Mg2+-ATPase activities in normal, regenerating and malignant cells. Biochim. Biophys. Acta, 1982, 684, 67–74.

  • Eibl, H. and Lands, W. E. M., A new, sensitive determination of phosphate. Anal. Biochem., 1969, 30, 51–57.

  • Tsakiris, S., Angelogianni, P., Schulpis, K. H. and Starridis, J. C., Protective effect of L-phenylalanine on rat brain acetylcholinesterase inhibition induced by free radicals. Clin. Biochem., 2000, 33, 103–106.

  • Olney, J. W., Collins, R. C. and Sloviter, R. S., Exotoxic mechanisms of epileptic brain damage. Adv. Neurol., 1986, 44, 857–877.

  • Lopez, E., Arce, C., Oset-Gasque, M. J., Canadas, S. and Gonzalez, M. P., Cadmium induces reactive oxygen species generation and lipid peroxidation in cortical neurons in culture. Free Rad. Biol. Med., 2006, 40, 940–951.

  • Tomlinson, G., Mutus, B. and McLennan, I., Activation and inactivation of acetylcholinesterase by metal ions. Can. J. Biochem., 1981, 59, 728–735.

  • Shukla, A., Shukla, G. S. and Srimal, R. C., Cadmium-induced alterations in blood-brain barrier permeability microvessel antioxidant potential in rat. Hum. Exp. Toxicol., 1996, 15, 400–405.

  • Sanui, H. and Rubin, H., The role of magnesium in cell proliferation and transformation. In Ions, Cell Proliferation and Cancer (eds Boyton, A. L., Mckochan, W. L. and Whil-Field, J. P.), Academic Press, New York, 1982, pp. 517–537.

  • Antonio, M. T., Corredor, L. and Leret, M. L., Study of the activity of several brain enzymes like markers of the neurotoxicity induced by perinatal exposure to lead and/or cadmium. Toxicol. Lett., 2003, 143, 331–340.

  • Santini, S. E., Basini, G., Bussolati, S. and Grasselli, F., The phytoestrogen quercetin impairs steroidogenesis and angiogenesis in swine granulosa cells in vitro. J. Biomed. Biotech., 2009, 2009, 1–8.

  • Pany, S., Pal, A. and Sahu, P. K., Neuroprotective effect of quercetin in neurotoxicity induced rats: role of neuroinflammation in neurodegeneration. Asian J. Pharm. Clin. Res., 2014, 7(4), 152–156.

  • Govind, P., Madhuri, S. and Verma, K. S., Antioxidant, immunomodulatory and anticancer activities of emblicaofficinalis: an overview. IRJP, 2011, 2(8), 38–42.

  • Dasaroju, S. and Gottumukkala, K. M., Current trends in the research of Emblica officinalis (Amla): a pharmacological perspective. Int. J. Pharm. Sci. Rev. Res., 2014, 24(2), 150–159.

  • Banerjee, J., Biswas, S., Madhu, N. R., Karmakar, S. R. and Biswas, S. J., A better understanding of pharmacological activities and uses of phytochemicals of Lycopodium clavatum: a review. J. Pharmacogn. Phytochem., 2014, 3(1), 207–210.

  • Sundaram, E. N. et al., Preliminary study to evaluate analgesic and behavioural effects of Lycopodium clavatum in experimental animals. IJRH, 2013, 7(4), 168–175.


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  • Ameliorative Effects of the Homeopathic Medicine Lycopodium 200c and Extract of Phyllanthus emblica in Cadmium-Induced Neurotoxicity in Mice

Abstract Views: 469  |  PDF Views: 131

Authors

Mahasweta Chatterjee
Genetics and Molecular Biology Unit, Department of Zoology, Vidyasagar University, Midnapore 721 102, India
Anupama De
Genetics and Molecular Biology Unit, Department of Zoology, Vidyasagar University, Midnapore 721 102, India
Gobinda Chandra Sadhukhan
Department of Zoology, Vidyasagar College, Kolkata 700 006, India
Jayanta Kumar Kundu
Genetics and Molecular Biology Unit, Department of Zoology, Vidyasagar University, Midnapore 721 102, India

Abstract


Cadmium is an extremely toxic heavy metal and causes neurotoxicity by inducing oxidative stress and membrane disturbances in brain. Phyllanthus emblica and Lycopodium 200c have anti-oxidative properties and are able to remove the cadmium-produced free radicals. This study investigates the role of Lycopodium 200c and Phyllanthus emblica (amlaki) in ameliorating the toxic effects of cadmium on the brain of mice. Swiss albino mice were used and divided into four different sets with one control, one induced, one with amlaki and other with both amlaki and Lycopodium treatment. To observe the changes, tests for brain acetylcholinesterase along with Mg2+ ATPase activities were performed. Results show that cadmium toxicity leads to decrease in enzymatic activities which can be reversed by the effects of amlaki and Lycopodium 200c.

Keywords


Antioxidative Properties, Cadmium, Free Radicals, Oxidative Stress, Toxicity.

References





DOI: https://doi.org/10.18520/cs%2Fv111%2Fi8%2F1368-1371