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Optimization of Key Factors for Enhanced Fermentative Biohydrogen Production from Water Hyacinth by RSM


Affiliations
1 School of Studies in Biotechnology, Pandit Ravishankar Shukla University, Raipur 492 010, India
 

This communication discusses the optimization of key factors for the enhanced bio-hydrogen production from water hyacinth. Three critical factors inoculums age (18-24 h), inoculums volume (20-80 ml/l) and concentration of sulphuric acid (0.5-2.0%) were optimized by response surface methodology (RSM) with central composite design (CCD) for better production. RSM analysis showed that all three factors significantly influenced hydrogen production. The optimum hydrogen production was 705 ml/l obtained with 21 h old bacterial culture, 50 ml/l inoculums with 1.25% sulphuric acid pre-treatment. The hydrogen concentration produced by Clostridium acetobutylicum NCIM 2877 was enhanced after using RSM. The results obtained indicate that RSM with CCD can be used as a technique to optimize culture conditions for enhancement of hydrogen production by pre-treatment of low-cost organic substrate; water hyacinth using dark fermentation methods may be one of the most promising approaches.

Keywords

Central Composite Design, Clostridium acetobutylicum NCIM 2877, Hydrogen Production, Response Surface Methodology, Water-Hyacinth.
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  • Optimization of Key Factors for Enhanced Fermentative Biohydrogen Production from Water Hyacinth by RSM

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Authors

Veena Thakur
School of Studies in Biotechnology, Pandit Ravishankar Shukla University, Raipur 492 010, India
Mona Tandon
School of Studies in Biotechnology, Pandit Ravishankar Shukla University, Raipur 492 010, India
S. K. Jadhav
School of Studies in Biotechnology, Pandit Ravishankar Shukla University, Raipur 492 010, India

Abstract


This communication discusses the optimization of key factors for the enhanced bio-hydrogen production from water hyacinth. Three critical factors inoculums age (18-24 h), inoculums volume (20-80 ml/l) and concentration of sulphuric acid (0.5-2.0%) were optimized by response surface methodology (RSM) with central composite design (CCD) for better production. RSM analysis showed that all three factors significantly influenced hydrogen production. The optimum hydrogen production was 705 ml/l obtained with 21 h old bacterial culture, 50 ml/l inoculums with 1.25% sulphuric acid pre-treatment. The hydrogen concentration produced by Clostridium acetobutylicum NCIM 2877 was enhanced after using RSM. The results obtained indicate that RSM with CCD can be used as a technique to optimize culture conditions for enhancement of hydrogen production by pre-treatment of low-cost organic substrate; water hyacinth using dark fermentation methods may be one of the most promising approaches.

Keywords


Central Composite Design, Clostridium acetobutylicum NCIM 2877, Hydrogen Production, Response Surface Methodology, Water-Hyacinth.

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DOI: https://doi.org/10.18520/cs%2Fv113%2Fi04%2F790-795