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Effects of Field Warming on a High Arctic Soil Bacterial Community:A Metagenomic Analysis


Affiliations
1 National Antarctic Research Centre and Institute of Biological Sciences, University of Malaya, 50603 Kuala Lumpur, Malaysia
2 British Antarctic Survey, NERC, High Cross, Madingley Road, Cambridge CB3 OET, United Kingdom
3 School of Science and Genomics Facility, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, 47500 Subang Jaya, Malaysia
 

Soil microbial communities in the Arctic, one of the most rapidly warming regions on Earth, play an important role in a range of ecological processes. This report describes initial studies of natural soil bacterial diversity at a High Arctic site on Svalbard, as part of a long-term field environmental manipulation study. The impact of increased soil temperature and water availability on soil microbial communities was investigated. The manipulation experiment, using open-top chambers, was installed in late summer 2014, and the soils were sampled soon after snow melt in July 2015. High throughput sequencing of 16S rRNA genes showed relatively uniform diversity across the study area and revealed no significant initial effect of treatments on bacterial communities over the first 10-month autumn–winter–spring manipulation period.

Keywords

Arctic Soil Bacterial Diversity, High Throughput Sequencing, Open Top Chambers, Svalbard.
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  • Symon, C., Arris, L. and Heal, B. (eds), Arctic Climate Impact Assessment, Cambridge University Press, Cambridge, UK, 2005.

  • Intergovernmental Panel on Climate Change, Climate Change 2014 – Impacts, Adaptation and Vulnerability: Regional Aspects, Cambridge University Press, 2014, pp. 3–5.

  • Cooper, E. J., Warmer shorter winters disrupt arctic terrestrial ecosystems. Annu. Rev. Ecol. Evol. Syst., 2014, 45, 271–295.

  • AMAP. Snow, Water, Ice and Permafrost in the Arctic (SWIPA): Climate Change and the Cryosphere. Arctic Monitoring and Assessment Programme (AMAP), Oslo, 2011.

  • Falkowski, P. G., Fenchel, T. and Delong, E. F., The microbial engines that drive Earth’s biogeochemical cycles. Science, 2008, 320, 1034–1039.

  • Vincent, W. F., Rae, R., Laurion, I., Howard-Williams, C. and Priscu, J. C., Transparency of Antarctic ice-covered lakes to solar UV radiation. Limnol. Oceanogr., 1998, 43, 618–624.

  • Henry, G. H. R. and Molau, U., Tundra plants and climate change: the International Tundra Experiment (ITEX). Glob. Chang. Biol., 1997, 3, 1–9.

  • Klindworth, A., Pruesse, E., Schweer, T. and Peplies, J., Quast, C., Horn, M. and Glöckner, F. O., Evaluation of general 16S ribosomal RNA gene PCR primers for classical and next generation sequencing based diversity studies. Nucleic Acids Res., 2013, 41, e1.

  • Caporaso, J. G. et al., QIIME allows analysis of high-throughput community sequencing data. Nat. Methods, 2010, 7, 335–336.

  • McDonald, D. et al., An improved greengenes taxonomy with explicit ranks for ecological and evolutionary analyses of bacteria and archaea. ISME J., 2012, 6, 610–618.

  • Ganzert, L., Bajerski, F. and Wagner, D., Bacterial community composition and diversity of five different permafrost-affected soils of Northeast Greenland. FEMS Microbiol. Ecol., 2014, 89, 426–441.

  • Marion, G. M. et al., Open-top designs for manipulating field temperature in high-latitude ecosystems. Glob. Chang. Biol., 1997, 3, 20–32.

  • Bokhorst, S. et al., Variable temperature effects of open top chambers at polar and alpine sites explained by irradiance and snow depth. Glob. Chang. Biol., 2012, 19, 64–74.

  • Walker, J. K. M., Egger, K. N. and Henry, G. H. R., Long-term experimental warming alters nitrogen-cycling communities but site factors remain the primary drivers of community structure in high Arctic tundra soils. ISME J., 2008, 2, 982–995.

  • Geml, J., Morgado, L. N., Semenova, T. A., Welker, J. M., Walker, M. D. and Smets, E., Long-term warming alters richness and composition of taxonomic and functional groups of arctic fungi. FEMS Microbiol. Ecol., 2015, 91, 1–13.


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  • Effects of Field Warming on a High Arctic Soil Bacterial Community:A Metagenomic Analysis

Abstract Views: 407  |  PDF Views: 139

Authors

P. P. J. Lim
National Antarctic Research Centre and Institute of Biological Sciences, University of Malaya, 50603 Kuala Lumpur, Malaysia
K. K. Newsham
British Antarctic Survey, NERC, High Cross, Madingley Road, Cambridge CB3 OET, United Kingdom
P. Convey
National Antarctic Research Centre and Institute of Biological Sciences, University of Malaya, 50603 Kuala Lumpur, Malaysia
H. M. Gan
School of Science and Genomics Facility, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, 47500 Subang Jaya, Malaysia
W. C. Yew
National Antarctic Research Centre and Institute of Biological Sciences, University of Malaya, 50603 Kuala Lumpur, Malaysia
G. Y. A. Tan
National Antarctic Research Centre and Institute of Biological Sciences, University of Malaya, 50603 Kuala Lumpur, Malaysia

Abstract


Soil microbial communities in the Arctic, one of the most rapidly warming regions on Earth, play an important role in a range of ecological processes. This report describes initial studies of natural soil bacterial diversity at a High Arctic site on Svalbard, as part of a long-term field environmental manipulation study. The impact of increased soil temperature and water availability on soil microbial communities was investigated. The manipulation experiment, using open-top chambers, was installed in late summer 2014, and the soils were sampled soon after snow melt in July 2015. High throughput sequencing of 16S rRNA genes showed relatively uniform diversity across the study area and revealed no significant initial effect of treatments on bacterial communities over the first 10-month autumn–winter–spring manipulation period.

Keywords


Arctic Soil Bacterial Diversity, High Throughput Sequencing, Open Top Chambers, Svalbard.

References





DOI: https://doi.org/10.18520/cs%2Fv115%2Fi9%2F1697-1700