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Analysis of in Vitro and in silico Anti-Hyperglycaemic Action of Bioflavonoids Isolated from Different Citrus Peels


Affiliations
1 Department of Pharmaceutical Technology, University College of Engineering, BIT Campus, Anna University, Tiruchirappalli 620 024, India
 

In this study, flavonoid-rich chloroform fraction (FRCF) of nine different citrus species was isolated by solvent fractionation technique and its effect on α- glucosidase, α-amylase, glucose uptake by yeast cells and glycosylation of haemoglobin was studied. FRCF of Citrus maxima was found to show higher activity in the in vitro assays and thus it was characterized by Electrospray Ionization Mass Spectrometry (ESI-MS) analysis. This led to the identification of icariin, hesperidin and diosmetin-6,8-di-C-glucoside. Their binding efficacy with targeted proteins such as glucokinase (GK), glycogen synthase kinases 3β (GSK 3β) and peroxisome proliferator-activated receptor-γ (PPAR-γ ) was studied by in silico analysis using Schrödinger Maestro software. Icariin exhibited maximum docking energy with GSK 3β, relatively less for GK and null for PPAR-γ. Hesperidin and diosmetin-6, 8-di-C-glucoside showed good binding affinity with PPAR-γ compared to GSK 3β.

Keywords

Anti-Hyperglycaemic Activity, Bioflavonoids, Citrus maxima, Yeast Cells.
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  • Analysis of in Vitro and in silico Anti-Hyperglycaemic Action of Bioflavonoids Isolated from Different Citrus Peels

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Authors

E. Sanmuga Priya
Department of Pharmaceutical Technology, University College of Engineering, BIT Campus, Anna University, Tiruchirappalli 620 024, India
P. Senthamil Selvan
Department of Pharmaceutical Technology, University College of Engineering, BIT Campus, Anna University, Tiruchirappalli 620 024, India
A. Syed Arshad
Department of Pharmaceutical Technology, University College of Engineering, BIT Campus, Anna University, Tiruchirappalli 620 024, India

Abstract


In this study, flavonoid-rich chloroform fraction (FRCF) of nine different citrus species was isolated by solvent fractionation technique and its effect on α- glucosidase, α-amylase, glucose uptake by yeast cells and glycosylation of haemoglobin was studied. FRCF of Citrus maxima was found to show higher activity in the in vitro assays and thus it was characterized by Electrospray Ionization Mass Spectrometry (ESI-MS) analysis. This led to the identification of icariin, hesperidin and diosmetin-6,8-di-C-glucoside. Their binding efficacy with targeted proteins such as glucokinase (GK), glycogen synthase kinases 3β (GSK 3β) and peroxisome proliferator-activated receptor-γ (PPAR-γ ) was studied by in silico analysis using Schrödinger Maestro software. Icariin exhibited maximum docking energy with GSK 3β, relatively less for GK and null for PPAR-γ. Hesperidin and diosmetin-6, 8-di-C-glucoside showed good binding affinity with PPAR-γ compared to GSK 3β.

Keywords


Anti-Hyperglycaemic Activity, Bioflavonoids, Citrus maxima, Yeast Cells.

References





DOI: https://doi.org/10.18520/cs%2Fv115%2Fi10%2F1928-1937