Current Science

Open Access Open Access  Restricted Access Subscription Access

Increased xylanase activity in Aspergillus niger through mutation


Affiliations
1 Department of Biotechnology, Invertis University, Bareilly 243 123, India
2 Department of Plant Sciences, MJP Rohilkhand University, Bareilly 243 006, India
 

Aspergillus niger is used for xylanase production on agricultural waste as substrate under broth culture. Rice straw and sugarcane bagasse have been the most potential substrate for xylanase production. Two different mutagens were used: UV radiation for different time durations and 5-bromouracil of different concentrations. Mutants so formed were selected on the basis of morphological and colony characteristics. Selected mutants were checked for their stability, requirement of amino acid and xylanase activity. Tested mutants showed 4-fold increase in xylanase activity from wild type

Keywords

Agricultural waste, mutation, rice straw, sugarcane bagasse, xylanase
User
Notifications
Font Size

  • Pathak, S. S., Sandhu, S. S. and Rajak, R. C. Mutation studies on fungal glucoamylase: a review. Int. J. Pharma Bio. Sci., 2005, 5, 297–308.

  • Polizeli, M. L. T. M. et al., Xylanases from fungi: properties and industrial applications. Appl. Microbiol. Biotechnol., 2005, 67, 577–591.

  • Solimon, H. M., Sherief, A. D. A. and Tanash. A. B. E. L., Production of xylanase by Aspergillus niger and Trichoderma viridae using some agricultural residues. Int. J. Agric. Res., 2012, 7(1), 46–57.

  • Bajpai, P., Application of enzymes in the pulp and paper industry. Biotechnol. Prog., 1999, 15, 147–157.

  • Angayarknni, J., Palaniswamy, M., Pradeep, B. V. and Swaminathan, K., Biochemical substitution of fungal xylanases for prebleaching of hardwood kraft pulp. Afr. J. Biotechnol., 2006, 5, 9291–9296.

  • Polizeli, M. L. T. M., Rizzatti, A. C. S., Terenzi, H. F., Jorge, J. A. and Amorium, D. S., Xylanases from fungi: properties and industrial applications. Appl. Microbiol. Biotechnol., 2005, 67, 577–591.

  • Betini, J. H. A., Michelin, M., Peixoto-Nogueira, S. C., Jorge, J. A., Terenzi, H. F. and Polizeli, M. L. T. M., Xylanases from Aspergillus niger, Aspergillus niveus and Aspergillus ochraceus produced under solid-state fermentation and their application in cellulose pulp bleaching; Bioproc. Biosyst. Eng., 2009, 32, 819– 824.

  • Waites, M. J., Morgan, N. L., John, S. R. and Higton, G., Industrial Microbiology – An Introduction, Wiley Blackwell Publishing, 2002.

  • Agrawal, R., Deepika, N. U. and Joseph, R., Strain improvement of Aspergillus sp. and Penecilllium sp. by induced mutation for biotransformation of alpha penine to verbinol. Biotechnol. Bioenerg., 1999, 63, 249–252.

  • Desai, D. I. and Iyer, B. D., Utilization of corn cob waste for cellulase-free xylanase production by Aspergillus niger DX-23: medium optimization and strain improvement. Waste Biomass Valorization, 2017, 8, 103–113.

  • Rowlands, R. T., Industrial strain improvement: mutagenesis and random screening procedure. Enzy. Microb. Technol., 1984, 6, 3–10.

  • Miller, G. L., Use of dinitrosalicylic acid reagent for determination of reducing sugar. Am. Chem. Soc. Anal. Chem., 1959, 31(3), 426–428.

  • Khan, A., Tremblay, D. and Le Duy, Assay of xylanase and xylosidase activities in bacterial and fungal cultures. Enzyme Microbiol. Technol., 1986, 8, 373–377.

  • Romanowska, I., Polak, J. and Bieleeki, S., Isolation and properties of Aspergillus niger IBT-90 xylanase for bakery. Appl. Microbiol. Biotechnol., 2006, 69, 665–671.

  • Kango, N., Agrawal, S. C. and Jain, P. C., Xylanase production by thermophilic fungi from soil and decomposing organic matter. Microbiol. Biotechnol. Sustain. Develop., 2004, B-13, 293–299.

  • Radha, S., Himakiran Babu, R., Sridevi, A., Prasad, N. B. L. and Narasimha, G., Development of mutant fungal strains of Aspergillus niger for enhanced production of acid protease in submerged and solid state fermentation. Eur. J. Exp. Biol., 2012, 2(5), 1517–1528.

  • Kirimura, K., Yagughi, T. and Usami, S., Intraspecific protoplast fusion of citric acid producing strains of Aspergillus niger. J. Ferment. Technol., 1986, 6, 473–479.

  • Elbouami, F., Isolation and regeneration of protoplasts from mycelium of Fusarium solani. Afr. Crop Sci. J., 2001, 9(2), 351–358.


Abstract Views: 283

PDF Views: 120




  • Increased xylanase activity in Aspergillus niger through mutation

Abstract Views: 283  |  PDF Views: 120

Authors

Anjali Sharma
Department of Biotechnology, Invertis University, Bareilly 243 123, India
A. K. Jaitly
Department of Plant Sciences, MJP Rohilkhand University, Bareilly 243 006, India
Pankaj Kumar Rai
Department of Biotechnology, Invertis University, Bareilly 243 123, India

Abstract


Aspergillus niger is used for xylanase production on agricultural waste as substrate under broth culture. Rice straw and sugarcane bagasse have been the most potential substrate for xylanase production. Two different mutagens were used: UV radiation for different time durations and 5-bromouracil of different concentrations. Mutants so formed were selected on the basis of morphological and colony characteristics. Selected mutants were checked for their stability, requirement of amino acid and xylanase activity. Tested mutants showed 4-fold increase in xylanase activity from wild type

Keywords


Agricultural waste, mutation, rice straw, sugarcane bagasse, xylanase

References





DOI: https://doi.org/10.18520/cs%2Fv121%2Fi7%2F966-968