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Increased xylanase activity in Aspergillus niger through mutation


Affiliations
1 Department of Biotechnology, Invertis University, Bareilly 243 123, India
2 Department of Plant Sciences, MJP Rohilkhand University, Bareilly 243 006, India
 

Aspergillus niger is used for xylanase production on agricultural waste as substrate under broth culture. Rice straw and sugarcane bagasse have been the most potential substrate for xylanase production. Two different mutagens were used: UV radiation for different time durations and 5-bromouracil of different concentrations. Mutants so formed were selected on the basis of morphological and colony characteristics. Selected mutants were checked for their stability, requirement of amino acid and xylanase activity. Tested mutants showed 4-fold increase in xylanase activity from wild type

Keywords

Agricultural waste, mutation, rice straw, sugarcane bagasse, xylanase
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  • Increased xylanase activity in Aspergillus niger through mutation

Abstract Views: 272  |  PDF Views: 111

Authors

Anjali Sharma
Department of Biotechnology, Invertis University, Bareilly 243 123, India
A. K. Jaitly
Department of Plant Sciences, MJP Rohilkhand University, Bareilly 243 006, India
Pankaj Kumar Rai
Department of Biotechnology, Invertis University, Bareilly 243 123, India

Abstract


Aspergillus niger is used for xylanase production on agricultural waste as substrate under broth culture. Rice straw and sugarcane bagasse have been the most potential substrate for xylanase production. Two different mutagens were used: UV radiation for different time durations and 5-bromouracil of different concentrations. Mutants so formed were selected on the basis of morphological and colony characteristics. Selected mutants were checked for their stability, requirement of amino acid and xylanase activity. Tested mutants showed 4-fold increase in xylanase activity from wild type

Keywords


Agricultural waste, mutation, rice straw, sugarcane bagasse, xylanase

References





DOI: https://doi.org/10.18520/cs%2Fv121%2Fi7%2F966-968