International Journal of Medical Sciences

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Jatamansi:A Source against Lung and Colon Cancer Cells


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1 Division of Biochemistry, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, Main Campus Chatha, JAMMU (J&K), India
     

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In vitro assay for cytotoxic activity of Nardostachys jatamansi has been carried out against eight human cancer cell lines from six different tissues via 95 per cent methanolic and aqueous extract at the concentration of 100 μg/ml using Sulphorhodamine blue (SRB) assay. Results revealed that methanolic extract from the stem leaves of N. jatamansi showed highest in vitro cytotoxic effect against four human cancer cell lines (A-549, COLO-205, SW-620, NCI-H322) from lung and colon origin. Based on in vitro data, it is suggested that further in vivo studies as well as identification of effective components from methanolic extract and their exact mechanism of action could be useful in designing new anticancer therapeutic agents.

Keywords

Nardostachys jatamansi, In vitro Cytotoxic, Cancer Cells, SRB Assay.
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  • Airi, S., Rawal, R. S., Dhar, U. and Purohit, A. N. (2000). Assessment of availability and habitat preference of jatamansi: a critically endangered medicinal plant of Western Himalayas. Curr. Sci. Banglore, 79: 1467-1470.

  • Ali, S., Ansari, K.A., Jafry, M.A., Kabir, H. and Devakar, G. (2000). Nardostachys jatamansi protects against liver damage induced by thioacetamide in rats. J. Ethnopharmacol., 71: 359-363

  • Bael, G.S., Kim, M.S., Park, K.C., Koo, B.S., Jo, I. J., Choi, S.B., Lee, D.S., Kim, Y.C., Kim, T.H., Seo, S.W., Shin, Y.K., Song, H.J. and Park, S.J. (2012). Effect of biologically active fraction of Nardostachys jatamansi on cerulein-induced acute pancreatitis. World J. Gastroenterol 18: 3223-3234.

  • Dhingra, D. and Goyal, P.K. (2008). Inhibition of MAO and GABA: Probable mechanisms for antidepressant-like activity of Nardostachys jatamansi DC. in mice. Indian J. Exp. Biol., 46: 212-218.

  • Kandil, O., Radwan, N. M., Hassan, A. B., Amer, A. A. M., El-banna, H. A. and Amer, W. M. M. (1994). Extracts and fractions of Thymus capitatus exhibit antimicrobial activities. J. Ethnopharmacol. 45: 97-111.

  • Lyle, N., Bhattacharyya, D., Sur, T. K., Munshi, S., Paul, S., Chatterjee, S. and Gomes, A. (2009). Stress modulating antioxidant effect of Nardostachys jatamansi. Indian J. Biochem. Biophys., 46: 93-98.

  • Monks, A., Scudiero, D., Skehan, P., Shoemaker, R., Paull, K., Vistica, D., Hose, C., Langley, J., Cronise, P., VaigroWolff, A., Gray-Goodrich, M., Campbell, H., Mayo, J. and Boyd, M. (1991). Feasibility of a high-flux anticancer drug screen using a diverse panel of cultured human tumor cell lines. J. Natl. Cancer Inst., 83: 57-66.

  • Pandita, R.M., Bhagat, M. and Saxena A.K. (2012). Evaluation of in vitro cytotoxicity of Nardostachys jatamansi ischolar_main extracts and fractions against neuroblastoma human cancer cell lines. J. Pharm. Res., 5: 2720-2722.

  • Salim, S., Ahmed, M., Zafar, K.S., Ahmad A. S. and Islam, F. (2003). Protective effect of jatamansi in rat cerebal ischemia. Pharmacol. Biochem. Behav., 74: 481-486.

  • Skehan, P., Storeng, R., Scudiero, D., Monks, A., McMohan, J., Vistica, D., Warren, J.T., Bokesch, H., Kenney, S. and Boyd, M.R. (1990). New colorimetric cytotoxicity assay for anticancer-drug screening. J. Natl. Cancer Inst., 82: 1107-1112.


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  • Jatamansi:A Source against Lung and Colon Cancer Cells

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Authors

Vikas Sharma
Division of Biochemistry, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, Main Campus Chatha, JAMMU (J&K), India

Abstract


In vitro assay for cytotoxic activity of Nardostachys jatamansi has been carried out against eight human cancer cell lines from six different tissues via 95 per cent methanolic and aqueous extract at the concentration of 100 μg/ml using Sulphorhodamine blue (SRB) assay. Results revealed that methanolic extract from the stem leaves of N. jatamansi showed highest in vitro cytotoxic effect against four human cancer cell lines (A-549, COLO-205, SW-620, NCI-H322) from lung and colon origin. Based on in vitro data, it is suggested that further in vivo studies as well as identification of effective components from methanolic extract and their exact mechanism of action could be useful in designing new anticancer therapeutic agents.

Keywords


Nardostachys jatamansi, In vitro Cytotoxic, Cancer Cells, SRB Assay.

References