International Journal of Plant Protection

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Evaluation of Various Aqua Suspension Formulations of Metarhizium anisopliae (Metschnikoff) Sorokin


Affiliations
1 Agricultural Research Station, Niphad, Nasik (M.S.), India
2 Department of Entomology, College of Agriculture, Latur (M.S.), India
     

   Subscribe/Renew Journal


Studies on evaluation of eleven aqua suspension formulations of entomopathogenic fungus, Metarhizium aniosopliae comprising 1) M.a.+ TW(0.5%) + CMC (0.5%) 2) M.a.+SFO(1.0%) + CMC(0.5%),3)M.a.+ SFO(1.0%)+HO(1.0%) 4) M.a.+ GNO(0.5%) + BA (2.0%),5) M.a.+ GNO(0.5%) + CMC(0.5%),6) M.a.+ GNO (0.5 %) + GH (0.5%), 7) M.a.+ GH (0.5%)+HO (1.0%), 8) Control (M.a.alone) were carried out. At 3 DAI, AS formulation with M.a.+GNO(0.5%)+CMC (0.5%) registered significantly highest (88.33%) surface coverage. The next promising formulations were M.a.+ SFO(1.0%) + CMC (0.5%) (78.33%),M.a.+ GNO (0.5%)+GH(0.5%) (56.67%) andM.a. + GH (0.5%) + HO (1.0%) (51.67%). Least (8.33%) growth of the fungus was recorded in M.a.+TW (0.5%)+CMC(0.5%); when, control recorded 30.0 per cent surface coverage. On 10 DAI, the differences (6.67 to 11.43g) for biomass production were significant. M.a. + SFO (1.0%)+CMC (0.5%) maintained its superiority over rest of the formulations by producing 11.43g biomass. However, it was at par with M.a.+SFO(1.0%)+HO(1.0) (11.10g). Maximum cfu count was observed inM.a.+SFO (1.0%)+ HO (1.0%) (2.33x109). However, it was at par withM.a.+SFO (1.0%) + CMC (0.5%) (2.27x109). The least (30.0%, 6.67g and 17.672x108 cfu/ml) surface coverage biomass and cfu count were recorded in control i.e. the formulation (M.a.alone), respectively. In case of bioefficacy of various formulations against larvae of Spodoptera litura, the formulation with M.a.+ SFO (1.0%) + HO(1.0%) registered significantly highest mortality of 80.00 and 73.33 per cent of II and III instar larvae of S.litura.

Keywords

Metarhizium aniosopliae, Media, Yeast Extract, Biomass, Colony Forming Unit, Spodoptera litura.
Subscription Login to verify subscription
User
Notifications
Font Size


  • Abbott,W.S. (1925). Method of computing effectiveness of an insecticide. J. Econ. Entomol., 18 : 265-267.

  • Alves, R.T., Oliveira, M.A.S., Batenan,R.P., Prior, C. and Leather, S.R. (2001). Spreading and effectiveness of an emulsifiable adjuvant oil fungal formulation. Bulletin de Pesquisa-e Desenvolvimento Embrapa Cerrados, No.6: 14 pp.

  • Aly, S.H. and Rashad, A.M. (1997). Effect of (Metarhizium anisopliae) on two stages of Earias insulana (Lepidoptera-Nectuidae). Analele Institutului de Cercetari pentru Cereale Protectia Plantelor, 28 (2) : 203-211.

  • Amer, M.M., El Sayad,T.I., Bakheit,K., Moustafa,S.A., El Sayad,Y.A. (2008). Pathogenicity and genitic variability of five entomopathogenic fungi against S.lituralis. Res. J. Agric. & Biological Sci., 4 (5) : 354-367.

  • Dayakar, S. and Kanaujia, K.R. (2001). Susceptibility of tobacco caterpillar, Spodoptera litura F.(Lepidoptera: Noctuidae) to different isolates of entomopathogenic fungi (Deuteromycotina : Hyphomycetes). Indian J. Plant Protec., 29 (1-2) : 62-67.

  • Dayakar, S. and Kanaujia, K.R. (2003). Evaluation of the pathogenicity of Beauveria bassiana, Metarhizium anisopliae and Nomuraea rileyi on different larval stages of tobacco caterpillar, Spodoptera litura (F.). Indian J. Plant Protect., 31: 9 - 12.

  • Dayakar, S. and Subbarao,Y. (2011). Influence of addition of host larval extract to medium on the virulence of Beauveria bassiana (Balsamo) Vuillemin and Metarhizium anisopliae (Metschnikoff) Sorokin against Spodoptera litura Fab. J. Biopesticides, 4 (1) : 91 - 95.

  • Gopalkrishnan,C. and Narayan, K. (1987). Studies on the dose, age relationship Metarhizium anisopliae (Metschikoff) Sorokin Var. anisopliae Tullock against Heliothis armigera Hub. (Noctuidae: Lepidoptera). Seminar cum sixth workshop on Biological control of crop pests and weeds. Gujrat Agricultural University, Anand (GUJARAT) INDIA.

  • Kaaya,G.P. and Hassan, N. (2000). Entomogenous fungi as promising biopesticides for tick control. Experiment.& Appl. Acarol., 24 (12) : 913-926.

  • Liu Bing Lan, Chen JechWei, Tzeng Yew Min, Liu, B.L., Chen,J.W. and Tzeng,Y.M. (2000). Production of cyclodepsipeptides destruxin A and B from Metarhizium anisopliae. Biotechnol. Prog., 16 (6) : 993-999.

  • Malsam,O., Kilian,M., Oerke,E.C. and Dehne, H.W. (2002). Oils for increased efficacy of Metarhizium anisopliae to control whiteflies. Biocontrol Sci. & Technol., 12 (3): 337-348.

  • McCoy,C.W, Samsom,R.A and Bourias, D.G. (1988). Entomogenous fungi, In “CRC Handbook of Natural pesticides, Microbial insecticide, part-A Entomogenous Protozoaand fungi,”C.M.Ignoffo,(Ed.), 5 : 151-236. Internat. J. Plant Protec., 10(2) Oct., 2017 : 262 HIND AGRICULTURAL RESEARCH AND TRAINING INSTITUTE

  • Metschnikoff, E. (1879). Diseases of larvae of grain weevil. Insects harmful to agriculture (series); Issue III, the grain weevil. Published by Commission attached to the Odessa Zemstvo Office for the investigation of the problem of insects harmful to agriculture. Odessa 32 pp.

  • Ming, Guang Feng, L.R., Johnso,J.B. and Kish,L.P. (1990). The virulence of Verticillium lecanii and an aphid derived isolate of Beauveria bassiana for six species of cereal infecting aphids. J. Environ. Entomol., 19 (3) : 815-820.

  • Pandey, A.K. and Kanaujia, K.R. (2004). Sensitivity of aphid, Lipaphis erysimi (Kalt) to Metarhizium anisopliae (Metschnikoff) Sorokin conidiospore. Appl. Biological Res., 6 (1/2) : 7-10.

  • Prior, C., Jollands, P. and Le Patourel,G. (1988). Infectivity of oil and water formulations of B.bassiana (Deutromycatina : Hyphomycetes) to the cocoa weevil pest Pantorhytes plutus (Coleoptera : Curculionidae). J. Invertebrate Pathol., 52 : 66-72.

  • Purwar,J.P. and Sachan,G.C. (2005).Biotoxicity ofBeauveria bassiana and Metarhizium anisopliae against Spodoptera litura and Spilarctia abliqua. Annl. Plant Protec. Sci., 13(2) : 360-364.

  • Ramoska,W.A. (1984). The influence of relative humidity on Beauvera bassiana infectivity and replication on the chinch bug, Blissus leucopterus. J. Invertebrate Pathol., 43 : 389-394.

  • Rodriguez Colorado,S.R., Rodriguez Maciel,J.C., Riestra Diaz,D., Villanueva Jimenez,J.A. and Rodriguez Lagunes, D.A. (2002). Influence of light and natural additives on the germination of Metarhizium anisopliae conidias. Manejo Integrado de Plagasy Agroecologia, 64 : 34-40.

  • Sharma, Shashi , Gupta, R.B.L., Yadava,C.P.S. and Sharma,S. (1998). Effect of temperatures on growth, sporulation and bioactivity of entomofungi against whitegrub (Holotrichia consanguinea). Indian J. Entomol., 60 (1) : 1-7.

  • Silva,W.O.B., Mitidieri,S., Schrank, A. and Vainstein, M.H. (2005). Production and extraction of an extracellular lipase from the entomopathogenic fungus Metarhizium anisopliae. Process Biochem., 40 (1) : 321-326.

  • Wiwat,C. (2004). Development of Nomuraea rileyi based biopesticide for controlling Lepidopteran larvae. Ph. D. Thesis, Mahidol University, Malaysia.


Abstract Views: 267

PDF Views: 0




  • Evaluation of Various Aqua Suspension Formulations of Metarhizium anisopliae (Metschnikoff) Sorokin

Abstract Views: 267  |  PDF Views: 0

Authors

S. D. Patil
Agricultural Research Station, Niphad, Nasik (M.S.), India
R. S. Jadhav
Department of Entomology, College of Agriculture, Latur (M.S.), India

Abstract


Studies on evaluation of eleven aqua suspension formulations of entomopathogenic fungus, Metarhizium aniosopliae comprising 1) M.a.+ TW(0.5%) + CMC (0.5%) 2) M.a.+SFO(1.0%) + CMC(0.5%),3)M.a.+ SFO(1.0%)+HO(1.0%) 4) M.a.+ GNO(0.5%) + BA (2.0%),5) M.a.+ GNO(0.5%) + CMC(0.5%),6) M.a.+ GNO (0.5 %) + GH (0.5%), 7) M.a.+ GH (0.5%)+HO (1.0%), 8) Control (M.a.alone) were carried out. At 3 DAI, AS formulation with M.a.+GNO(0.5%)+CMC (0.5%) registered significantly highest (88.33%) surface coverage. The next promising formulations were M.a.+ SFO(1.0%) + CMC (0.5%) (78.33%),M.a.+ GNO (0.5%)+GH(0.5%) (56.67%) andM.a. + GH (0.5%) + HO (1.0%) (51.67%). Least (8.33%) growth of the fungus was recorded in M.a.+TW (0.5%)+CMC(0.5%); when, control recorded 30.0 per cent surface coverage. On 10 DAI, the differences (6.67 to 11.43g) for biomass production were significant. M.a. + SFO (1.0%)+CMC (0.5%) maintained its superiority over rest of the formulations by producing 11.43g biomass. However, it was at par with M.a.+SFO(1.0%)+HO(1.0) (11.10g). Maximum cfu count was observed inM.a.+SFO (1.0%)+ HO (1.0%) (2.33x109). However, it was at par withM.a.+SFO (1.0%) + CMC (0.5%) (2.27x109). The least (30.0%, 6.67g and 17.672x108 cfu/ml) surface coverage biomass and cfu count were recorded in control i.e. the formulation (M.a.alone), respectively. In case of bioefficacy of various formulations against larvae of Spodoptera litura, the formulation with M.a.+ SFO (1.0%) + HO(1.0%) registered significantly highest mortality of 80.00 and 73.33 per cent of II and III instar larvae of S.litura.

Keywords


Metarhizium aniosopliae, Media, Yeast Extract, Biomass, Colony Forming Unit, Spodoptera litura.

References