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In vitro Evaluation of Fungicides, Bio-Control Agents and Plant Extracts against Early Blight of Tomato Caused by Alternaria solani (Ellis and Martin) Jones and Grout
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The present experiment was conducted to test the efficacy of fungicides, bio-control agents and plant extracts in vitro against Alternaria solani causing early blight of tomato. Seven fungicides viz., four systemic (Propiconazole, Azoxystrobin, Thiophanate methyl and Carbendazim) and three non-systemic (Mancozeb, Captan and Zineb) at four concentrations i.e. 50, 100, 150 and 200 ppm and seven plant extracts viz., Datura strumarium (Jimson weed), Allium sativum (Garlic), Azadirachta indica (Neem), Zingiber officinale (Ginger), Ocimum sanctum (Tulsi), Calotropis gigantea (Aak) and Eucalyptus chamadulonsis (Eucalyptus) also at four concentrations i.e. 5, 10, 15 and 20 per cent were evaluated through poison food technique. Seven bio-control agents viz., Trichoderma harzianum, T. viride, T. koningii, T. hamatum, T. atroviride, Aspergillus niger and A. flavus were also evaluated in this study through dual culture technique. Among the systemic fungicides, Propiconazole was proved to be highly effective and recorded cent per cent inhibition at their all concentrations while among the non-systemic, Mancozeb was proved to be effective at their all concentrations but recorded 100 per cent inhibition only at their higher concentration i.e. 400 ppm. Among different plant extracts used, Azadirachta indica (Neem) was significantly inhibit the mycelial growth of pathogen at all concentrations followed by Datura strumarium (Jimson weed) and Calotropis gigantea (Aak). Of all bio-control agents, highest inhibition of radial growth of test fungus was recorded in Trichoderma harzianum (80.37%) followed by T. viride (71.48%) and T. koningii (77.41 %). However, T. hamatum (27.41%) was least effective in this study.
Keywords
Fungicides, Bio-Control Agents, Plant Extracts against, Early Blight of Tomato, Alternaria solani.
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- Abada, K. A., Mostafa, S. H. and Mervat, R. (2008). Effect of some chemical salts on suppressing the infection by early blight disease of tomato. Egyptian J. Appl. Sci., 23: 47–58.
- Abdel-Sayed, M. H. F. (2006). Pathological, physiological and molecular variations among isolates of Alternaria solani the causal of tomato early blight disease: 181pp.
- Balanchard, D. (1992). A colour atlas of tomato diseases. Wolfe Publication Limited, Book House, London. p.298.
- Cherif, M. and Benhamou, N. (1990). Cytochemical aspects of chitin breakdown during the parasitic action of Trichoderma spp. on Fusarium oxysporum f. sp. radicis-lycopersici. Phytopathology, 80: 1406-1412.
- Chethana, B.S.,Ganeshan, G. , Rao, A.S. and Bellishree, K. (2012). In vitro evaluation of plant extracts, bioagents and fungicides against Alternaria porri (Ellis) Cif. Causing purple blotch disease of onion. Pest Mgmt. Hort. Ecosyst., 18 (2): 194-198.
- El-Abyad, M. S., El-Sayad, M. A., El-Shanshoury, A. R. and El-Sabbagh S. M. (1993). Towards the biological control of fungal and bacterial diseases of tomato using antagonistic Streptomyces spp. Plant Soil, 149:185–95.
- Ganie, S. A., Ghani, M. Y., Qazi Nissar and Shabir-u-Rehman (2013). Bioefficacy of plant extracts and biocontrol agents against Alternaria solani. African J. Microbiol. Res., 7(34): 4397 - 4402.
- Gomaa, A. M. I. (2001). Pathological studies on early blight of tomato. M.Sc. Thesis., Fac. Agric., Cairo University.
- Gondal, A. S., Ijaj, M., Riaj, K. and Khan, A. R. (2012).Effect of different doses of fungicide (Mancozeb) against Alternaria leaf blight of tomato in tunnel. J. Plant Pathol Microb., 3: 125.
- Hadian, S., Rahnama, K., Jamali, S. and Eskandari, A. (2011). Comparing neem extract with chemical control on Fusarium oxysporum and Meloidogyne incognita complex of tomato. Adv. Environ. Biol., 5(8): 2052-2057.
- Herle, G. S. and Kamanna, B.C. (2014). In vitro and in vivo evaluation of fungicides against early blight of potato caused by Alternaria solani (Ellis and Martin) Jones & Grout., 14. 971-975.
- Khafari, A.S., Bahraminejad, S. and Abbasi, S.(2014). Evaluation of the Anti- Alternaria solani activity of Allium hirtifolium Boiss. Pak. J. Bot., 46(2): 741-747.
- Kouyoumjian, R. E. (2007). Comparison of compost tea and biological fungicides for control of early blight in organic heirloom tomato production. M.Sc. Thesis, Clemson University, South Carolina 16-37pp.
- Nene, Y.L. and Thapliyal, P.N. (1979). Fungicides in plant disease control. 2nd Ed. Oxford and IBH Pub. Co., New Delhi, India.
- Pritesh, P. and Subramanian, R.B. (2011).PCR based method for testing Fusarium wilt resistance of tomato. African J. Basic & Appl. Sci., 3(5): 222-227.
- Saad, A.S.A., Kadous, E.A., Tayeb, E.H., Massoud, M.A., Ahmed S.M. and Abou El- Ela, A.S.A. (2014). The inhibitory effect of some antioxidants and fungicides on the growth of Alternaria solani and Fusarium solani in vitro. Middle east J. Agric. Res., 3(2): 123- 134.
- Thakur, S. and Harsh, N.S.K. (2014). Phylloplane fungi as biocontrol agent against Alternaria leaf spot disease of (Akarkara) Spilanthes oleracea. Bioscience Discovery, 5(2):139-144.
- Yanar, Y. A. Gokce, Kadioglu, H. Cam and Whalon, M. (2011). In vitro antifungal evaluation of various plant extracts against early blight disease (Alternaria solani) of potato. African J.Biotechnol., 10(42): 8291- 8295.
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