International Journal of Plant Sciences

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Effect of Different Additives for the Production of Lignocellulolytic Enzymes (Liquid Medium) in Volvariella volvacea


Affiliations
1 Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore (T.N.), India
2 Centre of Excellence for Millets, Athiyandal (T.N.), India
     

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Among the additives liquid culture amended with calcium carbonate was found to be superior for the production of exo β-1,4 glucanase (17.51 μ mol of glucose/ml), endo β-1,4 glucanase (18.39 μ mol of glucose/ml), β-glucosidase (23.21μ mol of p-nitrophenol/ml), xylanase (0.26 μ mol of xylose/ml), laccase (0.17 OD change/min/ml) and polyphenol oxidase (0.24 OD change/min/ml). This was followed by horse gram powder (16.83; 17.54; 20.95; 0.21; 0.14 and 0.21), calcium carbonate + gypsum (16.28; 17.07; 20.66; 0.20; 0.13 and 0.20) as against 13.33; 15.48; 19.05; 0.17; 0.10 and 0.17 in non-amended medium (control).

Keywords

Volvariella volvacea, Organic, Inorganic Additives, Enzyme Production, Liquid Medium.
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  • Effect of Different Additives for the Production of Lignocellulolytic Enzymes (Liquid Medium) in Volvariella volvacea

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Authors

A. Sudha
Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore (T.N.), India
M. Rajesh
Centre of Excellence for Millets, Athiyandal (T.N.), India

Abstract


Among the additives liquid culture amended with calcium carbonate was found to be superior for the production of exo β-1,4 glucanase (17.51 μ mol of glucose/ml), endo β-1,4 glucanase (18.39 μ mol of glucose/ml), β-glucosidase (23.21μ mol of p-nitrophenol/ml), xylanase (0.26 μ mol of xylose/ml), laccase (0.17 OD change/min/ml) and polyphenol oxidase (0.24 OD change/min/ml). This was followed by horse gram powder (16.83; 17.54; 20.95; 0.21; 0.14 and 0.21), calcium carbonate + gypsum (16.28; 17.07; 20.66; 0.20; 0.13 and 0.20) as against 13.33; 15.48; 19.05; 0.17; 0.10 and 0.17 in non-amended medium (control).

Keywords


Volvariella volvacea, Organic, Inorganic Additives, Enzyme Production, Liquid Medium.

References