International Journal of Plant Sciences

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Effect of Different Additives for the Production of Lignocellulolytic Enzymes (Liquid Medium) in Volvariella volvacea


Affiliations
1 Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore (T.N.), India
2 Centre of Excellence for Millets, Athiyandal (T.N.), India
     

   Subscribe/Renew Journal


Among the additives liquid culture amended with calcium carbonate was found to be superior for the production of exo β-1,4 glucanase (17.51 μ mol of glucose/ml), endo β-1,4 glucanase (18.39 μ mol of glucose/ml), β-glucosidase (23.21μ mol of p-nitrophenol/ml), xylanase (0.26 μ mol of xylose/ml), laccase (0.17 OD change/min/ml) and polyphenol oxidase (0.24 OD change/min/ml). This was followed by horse gram powder (16.83; 17.54; 20.95; 0.21; 0.14 and 0.21), calcium carbonate + gypsum (16.28; 17.07; 20.66; 0.20; 0.13 and 0.20) as against 13.33; 15.48; 19.05; 0.17; 0.10 and 0.17 in non-amended medium (control).

Keywords

Volvariella volvacea, Organic, Inorganic Additives, Enzyme Production, Liquid Medium.
Subscription Login to verify subscription
User
Notifications
Font Size


  • Ardon, O., Kerem, Z. and Harder, Y. (1996). Enhancement of laccase activity in liquid culures of the lignolytic fungus Pleurotus ostreatus by cotton stalk extract.J. biotechnol., 51: 201-207.

  • Arunprasad, M. (2004). Studies on potentiality of Lentinus connatus Berk. for bioconversion of sugarcane trash (Saccharum officinarum L.) into potential biomanure. M.Sc. (Ag.) Thesis, Tamil Nadu Agricultural University, Madurai (T.N.) India.

  • Bateman, D.F. (1964). Cellulase and Rhizoctonia disease of bean. Phytopathology, 54 : 1372-1377.

  • Bommaraju, P. (2002). Studies on Lignocellulolytic break down of certain agro-industrial wastes by Lentinus connatus Berk. for production of potential biomanure. M.Sc. (Ag.) Thesis, Tamil Nadu Agricultural University, Madurai (T.N.) India.

  • Chang, S.T. (1974). Production of straw mushroom (Volvariella volvacea) from cotton wastes. Mushroom J., 21: 384-353.

  • Chang, S.T. (1977). The origin and early development of straw mushroom cultivation. Economic Bot., 31: 374-376.

  • Chang, S.T. (1983). Prospect of V.volvacea cultivation. Newsletter for the tropics 4(2): 5-8.

  • Chang, S.T. and Miles, P.G. (1993). Edible mushrooms and their cultivation. CBS Publishers and Distributors, New Delhi. pp.240.

  • Frochner, S.C. and Eriksson, K.E. (1974). Induction of Neurospora crassa laccase with protein synthesis inhibitors. J. Bacteriol., 120 : 450-457.

  • Krishnamoorthy, A.S., Thiribhuvanamala, G., Shanthi, K. and Marimuthu, T. (2005). Outdoor cultivation of paddy straw mushroom as inter crop in maize field. Mushroom Res., 14 : 9-12.

  • Mandelb, M. (1975). Microbial sources of cellulose, In C. R. Wilke (ed.), Cellulose as a chemical and energy resource. Biotechnology and Bioengineering Symposium No. 5. John Wiley and Sons, Inc., New York. p. 81-105.

  • Miller, G. L. (1972). Use of dinitro salicylic acid reagent for determination of reducing sugar. Anal. Chem., 31: 426 - 428.

  • Munoz, Guillen, F., Martinez, A.T. and Martinez, M.J. (1997). Induction and chracterization of laccase in the lignolytic fungus Pleuotus eryingii. Curr. Microbiol., 34 : 1-5.

  • Natarajan, K. and Kaviyarasan,V. (1991). Changeas in extra cellular enzyme pattern in the substrate during growth and fruiting of Pleurotus citrinopileatus. Proc. Natl. symp. Mush., Thiruvanadapuram. pp.44.

  • Ng, T. K., Weimer, P.J. and Zeikus, J.G. (1977). Cellulolytic and physiological properties of Clostridium thermocellum. Arch. Microbiol. 114 : 1-7.

  • Sadasivam and Manickam, S. (1992). Biochemical methods. IInd Ed. pp. 5-187.

  • Saeed, M.S., Khan, M. and Imam-ul-haq, M. (1994). Growing paddy straw mushroom on water hyacinth . Pakistan J. Phytopathol., 6 : 130-134.

  • Su, U.T. and Seth, L. N. (1940). Cultivation of straw mushroom. Indian Farm, 1: 332-333.

  • Thakur, M.P. and Vijay, Y. (2006). Modern techniques of cultivating paddy straw mushroom in a commercial scale. Compendium of lectures-Emerging Areas in Mushroom Diversity, Production and Post Harvest Developments. pp.10-20.

  • Thomas, K.M., Ramakrishan, T.S. and Narsimhalu, I. L. (1943). Paddy straw mushroom. Madras Agric. J., 31: 57-59.

  • Wasite, R.L. (1961). Factors affecting competitive saprophytic colonization of the agar plate by various ischolar_main-infecting fungi. Trans.Br.Mycol.Soc., 44 : 145-159.


Abstract Views: 359

PDF Views: 0




  • Effect of Different Additives for the Production of Lignocellulolytic Enzymes (Liquid Medium) in Volvariella volvacea

Abstract Views: 359  |  PDF Views: 0

Authors

A. Sudha
Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore (T.N.), India
M. Rajesh
Centre of Excellence for Millets, Athiyandal (T.N.), India

Abstract


Among the additives liquid culture amended with calcium carbonate was found to be superior for the production of exo β-1,4 glucanase (17.51 μ mol of glucose/ml), endo β-1,4 glucanase (18.39 μ mol of glucose/ml), β-glucosidase (23.21μ mol of p-nitrophenol/ml), xylanase (0.26 μ mol of xylose/ml), laccase (0.17 OD change/min/ml) and polyphenol oxidase (0.24 OD change/min/ml). This was followed by horse gram powder (16.83; 17.54; 20.95; 0.21; 0.14 and 0.21), calcium carbonate + gypsum (16.28; 17.07; 20.66; 0.20; 0.13 and 0.20) as against 13.33; 15.48; 19.05; 0.17; 0.10 and 0.17 in non-amended medium (control).

Keywords


Volvariella volvacea, Organic, Inorganic Additives, Enzyme Production, Liquid Medium.

References