Journal of Environment and Sociobiology

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Cytochemistry of Cerebral Neurosecretory Cells in a Coleopterous Insect, Cybister Tripunctatus, with Special Reference to the Presence of Proteinic Materials


Affiliations
1 P. G. Dept. of Zoology, Ranchi University, Ranchi, India
     

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Neurosecretory cells (NSCs) have been identified as endocrinal unicellular glands in insects which secrete hormones responsible for the internal chemical coordination. With the help of the specific staining techniques, viz., Bargmann's Chrome-alum, Haematoxylin Phloxine (CHP) and Ewen's Paraldehyde Fuchsin (PF), the neurosecretory cells are distinctly recognized in the brain and the various ganglia of the ventral nerve cord of insects. These techniques not only provide topographical picture of the neurosecretory cell (NSC) but evaluate both, the qualitative and quantitative characteristic of the neurosecretory material so that different cell types and stages of activity of the neurosecretory system during physiological state can easily be recognized. The NSCs located in the brain of C. tripunctatus are classified according to their staining affinity and cytomorphological characteristics into four types, viz., A, B, C, and D types. Histochemical staining techniques applied for the demonstration of proteinic materials in NSCs located in cerebral complex of C. tripunctatus indicate that NSCs contain varied amount of SS - Proteins (Cystine-rich proteins), SH- Proteins (Cysteine- rich proteins), Tyrosine - rich Proteins and NH2-bound Proteins.

Keywords

Neurosecretory Cells, CHP & PF, Histochemical, Cybister Tripunctatus., Proteinic Materials.
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  • Abraham, A. 1966. The influence of environmental factors on the neurosecretory activity of the water beetle (Dytiscus marginalis). Revue Roum. Biol. (Ser. Zool.), 11: 25-33.

  • Anwar, I. and Ismail, S. 1979. Neurosecretory centers in the brain of adult Gryllus bimaculatus (De Geer) (Orthoptera: Gryllidae). Int. J. Insect Morphol. & Embryol., 8(5): 265-275.

  • Anwar, M. 1983. A histological study on the neuroendocrinal system of a coleopterous insect, Cybister tripunctatus africanus Cast. Qatar Univ. Sci. Bull., 3:181-197.

  • Arvy, L. and Gabe, M. 1962. Histochemistry of the neurosecretory product of the parsintercerebralis of pterygote insects. In: Neurosecretion. Ed. H. Heller R.B. Clark, pp. 331-344.

  • Banhawy, M. and Anwar, I. 1971. The neurosecretory cycle and neurosecretory substance in Gryllotalpa gryllotalpa (L). Ann. Zool. Acad. India, 7(3): 41-64.

  • Banhawy, M. and Anwar. I., 1981. The neurosecretory cells and neurosecretions in the developmental stages of the cotton leafworm – Spodoptera littoralis (b). Qatar Univ. Sc. Bull., 1: 153-167.

  • Barde, S. P. 1981. Neurosecretory cells in the optic lobe of two aquatic coleopteran species Dineutes indicus and Cybister rugulosus. Hydrobiologia, 76(1/2): 97-102.

  • Fletcher, B. S. 1969. The diversity of cell types in the neurosecretory system of the beetle Blaps mucronata. J. Insect Physiol., 15: 119-134.

  • Furtado, A. F. 1976. Etude histophysiologique des cellules neurosecretrices de la pars-intercerebralis des leaves femelle de Panstrongulus medistus (Heteroptera, Reduvlidae). C. R. Hebd. Seances Acad. Sci. Paris, 283: 163-273.

  • Ganguly, D. N. and Basu, B. D. 1962. Studies on some chemical contents of the neurosecretory cells of adult silk worm Bombyx mori L. Acta Histochem., 13: 34-46.

  • Girardie, A. and Girardie, J., 1967. Etude histolgique, histochimiqueet ultrastructurale de la pars intercerebralis chez Locusta migratoria L. (Orthoptera). Zeitschrift fur Zellorchngg, 78: 54 – 75.

  • Govardhan, T. L., Shyamasundary, K. and Rao, K. H. 1978. The structure and cytochemistry of the neurosecretory cells in the cerebral and ventral ganglia of Hydrous triangularis Say (Insecta: Coleoptera). Bull. Zool., 45: 307-314.

  • Gundevia, H. S. and Ramamurty, P. S. 1972. Histological studies of the neurosecretory and retrocerebral complex of the water beetle, Hydrophilus olivaceus Fabr. (Insecta: Coleoptera). Z. Morphol., 71(4): 355-375.

  • Highnam, K. C. 1976. Neurosecretory activity in Locusts, General and Comparative Endocrinology, 26(2): 74-78.

  • Indurkar, U. S. and Tembhare, D. B. 2012. Histomorphological studies on the neurosecretory cells in the brain of the beetle Cybister tripunctatus Ol. (Coleoptera: Dytiscidae). The Bioscan, 7(3): 409-413.

  • Novak, V. J. A. 1966. Insect hormones. 2nd Edition. Methuen & Co. Ltd., London. 478 pp.

  • Padmaja, M., Deecaraman, M. and Jaganthbose, M. T. 2010. Study of neurosecretory cells in Sand Lobster Thenus orientalis of Royapuram coast, Chennai. World Journal of Fish and Marine Sciences, 2(2): 82-85.

  • Panov, A. A. 1978. Neurosecretory cells in the pars intercerebralis of Orthoptera. Zool. Anz. Jena 201(1/2), S. 49-63.

  • Panov, A. A. and Melnikova, E. J. 1986. The cerebral neurosecretory cells and corpora cardiac of the longicorn beetles (Coleoptera: Cerambycidae). Zool. J. Anat., 114: 205-219.

  • Pawar, K. R. 2008. Histomorphological structure of the cephalic neuroendocrine system in the water beetle, Cybister tripunctatus Ol. (Coleoptera: Dytiscidae). J. ent. Res., 32(4): 283-290.

  • Pipa, R. L. 1961. Studies on the hexapod nervous system. IV. A cytological and histochemical study of neurons and their inclusions in the brain of cockroach.Periplaneta americana (L.). Biol. Bull., 121: 521-534.

  • Prasad, O. 1979. Studies on the neurosecretory system of three aquatic beetles : Hydrous indicus, Cybister limbatus and Sandrocottus dejeani (Coleoptera). J. Hirnforsch., 20(6): 657-61.

  • Prento, P. 1972. Histochemistry of neurosecretion in the pars intercerebralis, corpus cardiacum system of the desert locust, Schistocerca gregaria. General and Comparative Endocrinology, 18: 482-500.

  • Rehm, M. 1955. Morphologische and histochemische Untersuchungen neurosekret orischer Zellen Von Schmetterlinger. Z. Zellforsch., 42: 19-58.

  • Scharrer, E. 1966. Principles of neuroendocrine integration. Ass. Res. Nerv. Ment. Dis. Res. Publ., 43: 1-35.

  • Schreiner, B. 1966. Histochemistry of the A-cell neurosecretory material in the milk weed bug, Oncopeltus fasciatus Dallas (Heteroptera, Lygaeidae), with a discussion of the neurosecretory material, Gen. comp. Endocr., 6: 388-400.

  • Sidhra, D. V., Thakare, V. K. and Tembhare, D. B. 1983. The cephalic neuroendocrine system of the beetle, Mylabris pustulata (Thunb). (Meloidae: Coleoptera). J. Hirnforsch., 24: 71-78.

  • Sloper, J. 1957. Presence of a substance rich in protein-bound cystine or cysteine in the neurosecretory system of an insect. Nature, 179: 148-149.

  • Tembhare, D. B. 2008. Techniques in Life Sciences. First edition. Himalaya Publishing House, Mumbai.

  • Tembhare, D. B. and Paliwal, G. N. 1990. Cephalic neuroendocrine system of the rock honey bee, Apis dorsata F. worker. Proc. 11th International Congr., IUSSI, pp. 137-138.

  • Thomsen, E., 1965. The neurosecretory system of the adult Calliphora erythrocephala. II-Histology of the neurosecsretory cells of the brain and some related structures. Z. Zellforsch., 67: 693-717.

  • Wigglesworth, V. B. 1959. The histology of the nervous system of an insect, Rhodnius prolixus (Hemiptera). I-peripheral nervous system, Quart. J. Micr. Sci., 100: 285-298.

  • Wigglesworth, V. B. 1964. Advances in Insect Physiology. 1st Edition, Academic Press, Elsevier, London, 2: 247-336.


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  • Cytochemistry of Cerebral Neurosecretory Cells in a Coleopterous Insect, Cybister Tripunctatus, with Special Reference to the Presence of Proteinic Materials

Abstract Views: 343  |  PDF Views: 2

Authors

Natick Imam Mallick
P. G. Dept. of Zoology, Ranchi University, Ranchi, India

Abstract


Neurosecretory cells (NSCs) have been identified as endocrinal unicellular glands in insects which secrete hormones responsible for the internal chemical coordination. With the help of the specific staining techniques, viz., Bargmann's Chrome-alum, Haematoxylin Phloxine (CHP) and Ewen's Paraldehyde Fuchsin (PF), the neurosecretory cells are distinctly recognized in the brain and the various ganglia of the ventral nerve cord of insects. These techniques not only provide topographical picture of the neurosecretory cell (NSC) but evaluate both, the qualitative and quantitative characteristic of the neurosecretory material so that different cell types and stages of activity of the neurosecretory system during physiological state can easily be recognized. The NSCs located in the brain of C. tripunctatus are classified according to their staining affinity and cytomorphological characteristics into four types, viz., A, B, C, and D types. Histochemical staining techniques applied for the demonstration of proteinic materials in NSCs located in cerebral complex of C. tripunctatus indicate that NSCs contain varied amount of SS - Proteins (Cystine-rich proteins), SH- Proteins (Cysteine- rich proteins), Tyrosine - rich Proteins and NH2-bound Proteins.

Keywords


Neurosecretory Cells, CHP & PF, Histochemical, Cybister Tripunctatus., Proteinic Materials.

References