Journal of Environment and Sociobiology

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Cytochemistry of Cerebral Neurosecretory Cells in a Coleopterous Insect, Cybister Tripunctatus, with Special Reference to the Presence of Proteinic Materials


Affiliations
1 P. G. Dept. of Zoology, Ranchi University, Ranchi, India
     

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Neurosecretory cells (NSCs) have been identified as endocrinal unicellular glands in insects which secrete hormones responsible for the internal chemical coordination. With the help of the specific staining techniques, viz., Bargmann's Chrome-alum, Haematoxylin Phloxine (CHP) and Ewen's Paraldehyde Fuchsin (PF), the neurosecretory cells are distinctly recognized in the brain and the various ganglia of the ventral nerve cord of insects. These techniques not only provide topographical picture of the neurosecretory cell (NSC) but evaluate both, the qualitative and quantitative characteristic of the neurosecretory material so that different cell types and stages of activity of the neurosecretory system during physiological state can easily be recognized. The NSCs located in the brain of C. tripunctatus are classified according to their staining affinity and cytomorphological characteristics into four types, viz., A, B, C, and D types. Histochemical staining techniques applied for the demonstration of proteinic materials in NSCs located in cerebral complex of C. tripunctatus indicate that NSCs contain varied amount of SS - Proteins (Cystine-rich proteins), SH- Proteins (Cysteine- rich proteins), Tyrosine - rich Proteins and NH2-bound Proteins.

Keywords

Neurosecretory Cells, CHP & PF, Histochemical, Cybister Tripunctatus., Proteinic Materials.
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  • Cytochemistry of Cerebral Neurosecretory Cells in a Coleopterous Insect, Cybister Tripunctatus, with Special Reference to the Presence of Proteinic Materials

Abstract Views: 361  |  PDF Views: 2

Authors

Natick Imam Mallick
P. G. Dept. of Zoology, Ranchi University, Ranchi, India

Abstract


Neurosecretory cells (NSCs) have been identified as endocrinal unicellular glands in insects which secrete hormones responsible for the internal chemical coordination. With the help of the specific staining techniques, viz., Bargmann's Chrome-alum, Haematoxylin Phloxine (CHP) and Ewen's Paraldehyde Fuchsin (PF), the neurosecretory cells are distinctly recognized in the brain and the various ganglia of the ventral nerve cord of insects. These techniques not only provide topographical picture of the neurosecretory cell (NSC) but evaluate both, the qualitative and quantitative characteristic of the neurosecretory material so that different cell types and stages of activity of the neurosecretory system during physiological state can easily be recognized. The NSCs located in the brain of C. tripunctatus are classified according to their staining affinity and cytomorphological characteristics into four types, viz., A, B, C, and D types. Histochemical staining techniques applied for the demonstration of proteinic materials in NSCs located in cerebral complex of C. tripunctatus indicate that NSCs contain varied amount of SS - Proteins (Cystine-rich proteins), SH- Proteins (Cysteine- rich proteins), Tyrosine - rich Proteins and NH2-bound Proteins.

Keywords


Neurosecretory Cells, CHP & PF, Histochemical, Cybister Tripunctatus., Proteinic Materials.

References