Simultaneous Quantitation of Lansoprazole and Domperidone in Pharmaceutical Dosage by Liquid Chromatography

A simple and precise HPLC method has been developed for simultaneous quantitation of lansoprazole and domperidone in pharmaceutical formulations. The chromatographic separation was achieved at ambient temperature using an Intersil C₁₈ (4.6 × 250 mm, 10 μm) column using a mobile phase comprising of 0.1 M sodium acetate and acetonitrile (55: 45 v/v), pH adjusted to 7.0 with orthophosphoric acid. The retention times for lansoprazole and domperidone were 5.78 min and 3.77 min respectively. The mean recoveries from the capsule formulations were between 99-101%. The detector response was found to be linear in the concentration range of 24-36 μg/mL for lansoprazole and 8-12 μg/mL for domperidone. The developed method herein described can be employed for quality control and routine analysis of drugs in capsule formulation.