Quantitative Determination of Lafutidine in Tablets by First Order Derivative UV-Spectrophotometry Using Area under Curve

Lafutidine (LTD) is used as antiulcer and mucosal protective. A new simple, economical and rapid UVSpectrophotometric first order derivative method using 'Area Under Curve' (AUC) technique has been developed for the quantitative determination of LTD in bulk and tablets. Methanol (20% v/v) was used as a solvent. Zero order spectrum of LTD was derivatised into first order using UV-probe software of the UVVis Spectrophotometer and the AUC was determined between the two selected wavelength 276.80 nm to 306.00 nm. LTD followed linearity in the concentration range of 10 - 80 μg/mL with r² > 0.99. The quantity of drug estimated by this method was in good accord with label claimed. This method was found to be accurate, precise and ruggedness as shown by low values of % RSD.