Comparative In Vitro Antimicrobial Activity of Three Different Macro Fungus Extracts against Clinical Isolates of Gram-Negative Pathogens

The three species of edible mushrooms fistulina hepatica, cantharellus cibarius, lepistanuda were collected from the Western Ghats and malnad forest area of Karnataka. Methanolic extracts of these three edible mushrooms were used in this study. Among the isolated mushrooms fistulina hepatica and cantharellus cibarius were showed satisfactory results. Determination of antimicrobial activity profile of all the isolates tested against a panel of standard pathogenic bacteria indicated that the concentrations of bioactive components directly influence the antimicrobial capability of the isolates. Agar diffusion assay showed considerable activity against all bacteria. These results are discussed in relation to therapeutic value of the studied mushrooms using the cup plate and paper disc methods. Gram negative microorganisms studied were Escherichia coli, Solmonella typhi, Morexella, H.influenza. Neisseria gonorrhoeae.

For examination of pathogenic microorganisms in the cup plate and disc diffusion method, methanolic extracts of concentrations of 250 and 500 micrograms per cup and disc were used. All the three extracts have demonstrated significant antimicrobial activities in both methods.