Research Journal of Pharmacology and Pharmacodynamics

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Chemopreventive Potential of Aegle marmelos Fruit Extract against 7,12-dimethylbenz(a)Anthracene-Induced Skin Papillomagenesis in Mice


Affiliations
1 Jawaharlal Nehru Cancer Hospital and Research Centre, Idgah hills, Bhopal (MP), India
2 Deptt. of Biosciences, Barkatullah University, Bhopal (MP), India
3 Columbia Institute of Pharmacy, Raipur, India
     

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The present investigation was undertaken to explore the chemopreventive action of Aegle marmelos fruit extract on 2-stage carcinogenesis, induced by a single topical application of 7, 12-Dimethylbenz(a)anthracene (DMBA) (104μg/100μl acetone), and one week later, promoted by repeated application of croton oil (1% in acetone/twice in a week) till the end of the experiment (16 weeks) in Swiss albino mice. Single topical application of Aegle marmelos fruit extract at a dose of 900 mg/kg b.w. along with DMBA+Croton oil was found to be effective in decreasing the cumulative number of papillomas, tumor incidence, tumor yield and tumor burden as compared to control group (p<0.05). The depleted levels of glutathione were also restored in Aegle marmelos fruit extract treated group. Thus, the present study revealed the chemopreventive role of Aegle marmelos fruit extracts against DMBA-induced skin carcinogenesis in mice.

Keywords

Chemopreventive, Aegle marmelos, DMBA, Papilloma, Skin Carcinogenesis, Glutathione.
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  • Arul V, Miyazaki S and Dhananjayan R (2005): Studies on the anti-inflammatory, antipyretic and analgesic properties of the leaves of Aegle marmelos Correa. Journal of Ethnopharmacology, 96(1), 159- 163.

  • Arumugam S, Kavimani S, Kadalmani B, Ahmed ABA, Akbarsha MA, Rao MV (2008): Antidiabetic activity of leaf and callus extracts of Aegle marmelos in rabbit. Science Asia, 34, 317-321.

  • Ather M (2002): Oxidative stress and experimental carcinogenesis. Indian J Exp Biol, 40, 656-667.

  • Agrawal RC, Jain R, Raja W and Ovais M (2009): Anticarcinogenic effects of Solanum lycopersicum fruit extract on Swiss albino and C57BL mice. Asian Pacific Journal of Cancer prevention, 10(1-4), 379-382.

  • Berenblum I and Shubik P (1947): A new quantitative approach to the study of stages of Chemical carcinogenesis in the mouse skin. Brit J Cancer, 1, 383-387.

  • Berenblum I (1975): Sequqntial aspects of chemical carcinogenesis: skin in cancer “A comprehensive treatise”. Plenum Press, New York, 1, 323-344.

  • Beutler E, Duron O, Kellin BM (1963): Improved method for the determination of blood glutathione. Journal of Laboratory and Clinical Medicine, 61, 882-888.

  • Brijesh S, Daswani P, Tetali P, Anita N, Birdi T (2009): Studies on the antidiarrhoeal activity of Aegle marmelos unripe fruits: Validating its traditional usage. BMC complementary and Alternative medicine, 9(47), 1-12.

  • Bystrianyk R (2005): Aloe helps block cancer. J Toxicol Environ health, 17, 45-49.

  • Chaudhary G, Saini MR, Goyal PK (2007): Chemopreventive potential of Aloe vera against 7, 12-dimethylbenz(a)anthracene induced skin papillomagenesis in mice. Integrative cancer Therapies, 6(4), 405-412.

  • Copeland ES (1983): A national institute of health workshop report. Free radical in promotion. A chemical pathology study section workshop. Cancer Research, 43, 5631-5637.

  • Jagetia GC, Venkatesh P, Baliga MS (2005): Aegle marmelos (L.) Correa Inhibits the proliferation of Transplanted Ehrlich Ascites Carcinoma in mice. Biol. Pharm. Bulletin, 28(1), 58-64.

  • Lampronti I, Martello D, Bianchi N, Borgatti M, Lambertini E, Piva R, Jabbar S, Choudhari MS, Khan MT, Gambari R (2003): Antiproliferative effects on human tumor cell lines of extracts from the Bangladeshi medicinal plant Correa. Phytomedicine, 10(4), 300-308.

  • Moron MS, Depierre JW and Mannervik B (1979): Levels of Glutathione, Glutathione Reductase and Glutathione-s-transferase activities in rat lung and liver. Biochimica et Biophysica Acta, 582, 67-78.

  • Morse EC, Stoner G (1996): Cancer chemoprevention: principles and prospects. Carcinogenesis, 14, 1737-1746.

  • Lewis JG and Adams DO (1987): Early inflammatory changes in the skin of Sencer and C57B2/6 mice following exposure to 12-Otetradecanoyl phorbol-13-acetate. Carcinogenesis, 8, 889-898.

  • Nugroho AE, Riyanto S, Sukari MA, Maeyama K (2010): Effects of Skimmianine, a quinoline alkaloid of Aegle marmelos Correa ischolar_mains, on the Histamine release from rat mast cells. Journal of Basic and applied Sciences, 6(2), 141-148.

  • Oberley TD and Oberley LW (1993): Oxygen radicals and cancer. In free radicals in Ageing' EdYuBP, CRC Press, Boca Raton, FL, pg-247-267.

  • Narendra T, Shweta S, Tiwari P, Reddy KP, Khaliq T, Prathipati P, Puri A, Srivastava AK, Chander R, Agarwal SC, Raj K (2007): Antihyperglycemic and antidyslipidemic agent from Aegle marmelos. Bioorg Med Chem Letters, 17(6), 1808-1811.

  • Meena PD, Kaushik P, Shukla S, Soni AK, Kumar M, Kumar A (2006): Anticancer and antimutagenic properties of Acacia nilotica (Linn) on 7, 12-dimethylbenz(a)anthracene induced skin papillomagenesis in Swiss Albino mice. Asian pacific Journal of cancer prevention, 7, 627-632.

  • Sabu MC and Kuttan R (2004): Antidiabetic activity of Aegle marmelos and its relationship with its antioxidant properties. Indian J. Physiol Pharmacology, 48(1), 81-88.

  • Samarasekera I and Krishnamurti JK (2004): Isolation of various coumarin present in the various parts of the Aegle marmelos. Nat. Prod. Res., 18(2), 117-132.

  • Singanan V, Singanan M and Begum H (2007): The hepatoprotective effect of Bael leaves (Aegle marmelos) in alcohol induced liver injury in Albino rats. International Journal of Science and Technology, 2(2), 83-92.

  • Upadhya S, Shanbhag KK, G Suneetha, Naidu, Upadhya S (2004): A study of Hypoglycemic and antioxidant activity of Aegle marmelos in Alloxan induced diabetic rats. Indian Journal of Physiol Pharmacology, 48(4), 476-480.


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  • Chemopreventive Potential of Aegle marmelos Fruit Extract against 7,12-dimethylbenz(a)Anthracene-Induced Skin Papillomagenesis in Mice

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Authors

Nirmala Gupta
Jawaharlal Nehru Cancer Hospital and Research Centre, Idgah hills, Bhopal (MP), India
R. C. Agrawal
Jawaharlal Nehru Cancer Hospital and Research Centre, Idgah hills, Bhopal (MP), India
Vinoy Shrivastava
Deptt. of Biosciences, Barkatullah University, Bhopal (MP), India
Amit Roy
Columbia Institute of Pharmacy, Raipur, India
Pushpa Prasad
Columbia Institute of Pharmacy, Raipur, India

Abstract


The present investigation was undertaken to explore the chemopreventive action of Aegle marmelos fruit extract on 2-stage carcinogenesis, induced by a single topical application of 7, 12-Dimethylbenz(a)anthracene (DMBA) (104μg/100μl acetone), and one week later, promoted by repeated application of croton oil (1% in acetone/twice in a week) till the end of the experiment (16 weeks) in Swiss albino mice. Single topical application of Aegle marmelos fruit extract at a dose of 900 mg/kg b.w. along with DMBA+Croton oil was found to be effective in decreasing the cumulative number of papillomas, tumor incidence, tumor yield and tumor burden as compared to control group (p<0.05). The depleted levels of glutathione were also restored in Aegle marmelos fruit extract treated group. Thus, the present study revealed the chemopreventive role of Aegle marmelos fruit extracts against DMBA-induced skin carcinogenesis in mice.

Keywords


Chemopreventive, Aegle marmelos, DMBA, Papilloma, Skin Carcinogenesis, Glutathione.

References