Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Effect of Superoxide Dismutase Mimetic Tempol on Dexamethasone Induced Insulin Resistance- Role of Oxidative Stress


Affiliations
1 R.R.K.S College of Pharmacy, Bidar, Karnataka., India
2 S.V.E.T College of Pharmacy, Humnabad, Bidar Karnataka, India
3 Karnataka College of Pharmacy, Bidar Karnataka, India
     

   Subscribe/Renew Journal


Glucocorticoids like Dexamethasone are widely used Immunosuppressant and Anti-inflammatory agent. They have several adverse effects, majorly Insulin Resistance (IR) and Hypertension these are probably attributed to Oxidative Stress. In the present study, effect of SOD Mimetic Tempol (2,2,6,6,-tetramethyl-4-hydroxy-1-piperidinyloxy) was administered (1mmol/L) for Prophylactic as well as Therapeutic regimen in Experimental Induced Insulin Resistance in male Wister rats by using Dexamethasone (20μg/kg) subcutaneously and estimated Plasma Insulin and TBARS were measured in Plasma as well in isolated Aortic tissue as a marker of Oxidative Stress. HOMA-IR index and Mastuda Index were calculated as an Insulin Resistance/ Insulin Sensitivity. From the finding of present study, Tempol failed to produce any effect on IR so in conclusion, Oxidative Stress may be playing the Initiative role in Dexamethasone Induced IR.

Keywords

Insulin Resistance (IR), Oxidative Stress, Dexamethasone, Insulin Resistance Index (Homa- IR) And Insulin Sensitivity Index (Mastuda Index).
Subscription Login to verify subscription
User
Notifications
Font Size


  • Bjelakovic G, Beninati S, Pavlovic D., Glucocorticoids and oxidative stress,18(2): 115-27(2007).
  • Xi L, Qian Z, Shen X, Wen N, Zhang Y, Crocetin prevents dexamethasone-induced insulin resistance in rats. Planta Med. 71(10): 917-22 (2005).
  • Ogihara T, Asano T, Ando K, Yuko C, Sakoda H, Anai M, Shojima N, Angiotensin II–Induced Insulin Resistance Is Associated With Enhanced Insulin Signaling. Hypertension, 40: 872-79 (2002).
  • Rhee MS, Perianayagam A, Chen P, Youn JH, Dexamethasone treatment causes resistance to insulin-stimulated cellular potassium uptake in the rat. Am J Physiol Cell Physiol, 287:1229–37 (2004).
  • Britt E, Mette YD, Mulvany MJ, The superoxide dismutasemimetic, Tempol blunts right ventricular hypertrophy in chronic hypoxic rats, B J P, 141: 105–13 (2004).
  • Loraine JA, .Bell ET. Hormone assay and their clinical application. 4th ed. New York: Churchill livingstone. (1976).
  • Buccolo G, David H. Quantitative determination of serum triglycerides by use of Enzymes. Clin Chem, 19:476-82 (1973).
  • Henry RR. Insulin resistance: from predisposing factor to therapeutic target in type 2 diabetes. Clin Ther, 25(B): B47-63 (2003).
  • Albareda M, Espinosa JR, Murugo M, Leiva A, Corocoy R. Assessment of insulin sensitivity and beta-cell function from measurements in the fasting state and during an oral glucose tolerance test. Diabetol, 43: 4507-11(2000).
  • Stojanovska L, Rosella G and Proietto J. Evolution of dexamethasone-induced insulin resistance in rats. Am J Physiol, 258: 748–56 (1990).
  • Marwaha A, Mustafa F. Tempol reduces oxidative stress and restores renal dopamine D1-like receptor-G protein coupling and function in hyperglycemic rats. Am J Physiol Renal Physiol, 291: F58–66 (2006).
  • Ogihara T et al. Angiotensin II–Induced Insulin Resistance Is Associated With Enhanced Insulin Signaling. Hypertension, 40: 872-79(2002).
  • Yu W, Xu A, Ye J, Edward W et al. Alteration in Phosphorylation of P20 Is Associated With Insulin Resistance. Diabetes, 50: 1821–27 (2001).
  • Henry RR. Insulin resistance: from predisposing factor to therapeutic target in type 2 diabetes. Clin Ther, 25(B): B47-63 (2003).
  • Samy I, Mc F, Banerji M, and James RS. Insulin Resistance and Cardiovascular Disease. J Clini Endocrin Metabol, 86(2): 713- 18 (2001).
  • Denis J. Dysregulation of fatty acid metabolism in the etiology of type 2 diabetes. Diabetes, 51(1): 7-18(2002).

Abstract Views: 334

PDF Views: 2




  • Effect of Superoxide Dismutase Mimetic Tempol on Dexamethasone Induced Insulin Resistance- Role of Oxidative Stress

Abstract Views: 334  |  PDF Views: 2

Authors

Karnakumar V. Biradar
R.R.K.S College of Pharmacy, Bidar, Karnataka., India
Chandrashekhar B. Patil
R.R.K.S College of Pharmacy, Bidar, Karnataka., India
Basavaraj V. Chivde
S.V.E.T College of Pharmacy, Humnabad, Bidar Karnataka, India
M. H. Malipatil
Karnataka College of Pharmacy, Bidar Karnataka, India
Sanjivkumar D. Biradar
R.R.K.S College of Pharmacy, Bidar, Karnataka., India

Abstract


Glucocorticoids like Dexamethasone are widely used Immunosuppressant and Anti-inflammatory agent. They have several adverse effects, majorly Insulin Resistance (IR) and Hypertension these are probably attributed to Oxidative Stress. In the present study, effect of SOD Mimetic Tempol (2,2,6,6,-tetramethyl-4-hydroxy-1-piperidinyloxy) was administered (1mmol/L) for Prophylactic as well as Therapeutic regimen in Experimental Induced Insulin Resistance in male Wister rats by using Dexamethasone (20μg/kg) subcutaneously and estimated Plasma Insulin and TBARS were measured in Plasma as well in isolated Aortic tissue as a marker of Oxidative Stress. HOMA-IR index and Mastuda Index were calculated as an Insulin Resistance/ Insulin Sensitivity. From the finding of present study, Tempol failed to produce any effect on IR so in conclusion, Oxidative Stress may be playing the Initiative role in Dexamethasone Induced IR.

Keywords


Insulin Resistance (IR), Oxidative Stress, Dexamethasone, Insulin Resistance Index (Homa- IR) And Insulin Sensitivity Index (Mastuda Index).

References