The Asian Journal of Horticulture

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Green Synthesis and Characterization of Silver (Ag) Nanoparticles Using Neem Leaf Extract and its Antifungal Activity against Seed Borne Pathogens in Chilli


Affiliations
1 Department of Seed Science and Technology, Tamil Nadu Agricultural University, Coimbatore (T.N.), India
2 , Horticultural College and Research Institute for Women, Trichy (T.N.), India
3 Department of Floriculture, Tamil Nadu Agricultural University, Coimbatore (T.N.), India
     

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The silver nanoparticles (AgNPs) synthesized using hot water neen leaf extract (NLF) as reducing and stabilizing agent are reported and evaluated for antibacterial activity against chilli seed borne pathogens. The effect of extract concentration, contact time, pH and temperature on the reaction rate and the shape of the Ag nanoparticles were investigated. The data revealed that the rate of formation of the nanosilver size decreased significantly in the basic medium with different concentration (1 mM, 5 mM and 10 mM). Synthesized was characterized by UV-vis spectroscopy, X-ray diffraction, scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The nanoparticle synthesis using different concentration of silver nitrate and 5 mM was getting nano sized silver particles AgNPs. The silver nanoparticles were with an average size of 55 to 350 nm and mostly rod shape. The antifungal activity of synthesized AgNPs was observed in seed health test and different concentration of silver NPs (750 mg, 1000 mg and 1250 mg) were used for seed health test. The AgNPs at 750 mg concentration significantly inhibited the seed borne pothogens. Thus AgNPs showed broad spectrum antifungal activity at lower concentration and may be a good alternative therapeutic approach in future.

Keywords

Green Synthesis, Silver Nanoparticles, Neem Leaf Extract, Antifungal Activity.
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  • Green Synthesis and Characterization of Silver (Ag) Nanoparticles Using Neem Leaf Extract and its Antifungal Activity against Seed Borne Pathogens in Chilli

Abstract Views: 271  |  PDF Views: 0

Authors

G. Dileep Kumar
Department of Seed Science and Technology, Tamil Nadu Agricultural University, Coimbatore (T.N.), India
N. Natarajan
, Horticultural College and Research Institute for Women, Trichy (T.N.), India
M. Divya
Department of Floriculture, Tamil Nadu Agricultural University, Coimbatore (T.N.), India

Abstract


The silver nanoparticles (AgNPs) synthesized using hot water neen leaf extract (NLF) as reducing and stabilizing agent are reported and evaluated for antibacterial activity against chilli seed borne pathogens. The effect of extract concentration, contact time, pH and temperature on the reaction rate and the shape of the Ag nanoparticles were investigated. The data revealed that the rate of formation of the nanosilver size decreased significantly in the basic medium with different concentration (1 mM, 5 mM and 10 mM). Synthesized was characterized by UV-vis spectroscopy, X-ray diffraction, scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The nanoparticle synthesis using different concentration of silver nitrate and 5 mM was getting nano sized silver particles AgNPs. The silver nanoparticles were with an average size of 55 to 350 nm and mostly rod shape. The antifungal activity of synthesized AgNPs was observed in seed health test and different concentration of silver NPs (750 mg, 1000 mg and 1250 mg) were used for seed health test. The AgNPs at 750 mg concentration significantly inhibited the seed borne pothogens. Thus AgNPs showed broad spectrum antifungal activity at lower concentration and may be a good alternative therapeutic approach in future.

Keywords


Green Synthesis, Silver Nanoparticles, Neem Leaf Extract, Antifungal Activity.

References