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Green Synthesis and Characterization of Silver (Ag) Nanoparticles Using Neem Leaf Extract and its Antifungal Activity against Seed Borne Pathogens in Chilli
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The silver nanoparticles (AgNPs) synthesized using hot water neen leaf extract (NLF) as reducing and stabilizing agent are reported and evaluated for antibacterial activity against chilli seed borne pathogens. The effect of extract concentration, contact time, pH and temperature on the reaction rate and the shape of the Ag nanoparticles were investigated. The data revealed that the rate of formation of the nanosilver size decreased significantly in the basic medium with different concentration (1 mM, 5 mM and 10 mM). Synthesized was characterized by UV-vis spectroscopy, X-ray diffraction, scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The nanoparticle synthesis using different concentration of silver nitrate and 5 mM was getting nano sized silver particles AgNPs. The silver nanoparticles were with an average size of 55 to 350 nm and mostly rod shape. The antifungal activity of synthesized AgNPs was observed in seed health test and different concentration of silver NPs (750 mg, 1000 mg and 1250 mg) were used for seed health test. The AgNPs at 750 mg concentration significantly inhibited the seed borne pothogens. Thus AgNPs showed broad spectrum antifungal activity at lower concentration and may be a good alternative therapeutic approach in future.
Keywords
Green Synthesis, Silver Nanoparticles, Neem Leaf Extract, Antifungal Activity.
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- Bhattacharya, D. and Gupta, R.K. (2005). Nanotechnology and potential of microorganisms. Crit. Rev. Biotechnol., 25 : 199-204
- Brett, D.W. (2006). A discussion of silver as an antimicrobial agent: alleviating the confusion. Ostomy Wound Manage, 52: 34-41.
- Farooqui M.A, Chauhan, P.S., Krishnamoorthy, P. and Shaik, J. (2010). Digest. J. Nanomater Biostruct., 5: 43.
- ISTA (2010). International rules of seed testing. Seed Sci. & Technol., 11 : 20-35.
- Kalmesh, M. and Gurjar, R.B.S. (2001). Sclerotium rolfsii – A new threat to chilli in Rajasthan. Mycol. & Plant Pathol., 31(2) : 261
- Lee, P.C. and Meisel, D. (2005). Adsorption and surfaceenhanced raman of dyes on silver and gold sols. J. Physics Chem., 86 : 3391-3395
- Min, J.S. (2009). Effects of colloidal silver nanoparticles on sclerotium-forming phytopathogenic fungi. Plant Pathol., 25: 376–380.
- Mohanpuria, P., Rana, N.K. and Yadav, S.K. (2008). Biosynthesis of nanoparticles: technological concepts and future applications. J. Nanopart Res., 10 : 507-517
- Park, H.J., Kim, S.H., Kim, H.J. and Choi, S.H. (2006). A new composition of nano sized silica-silver for control of various plant diseases. Plant Pathol. J., 22 : 295–302.
- Toraya, H. (1986). Whole- powder- pattern fitting without reference to structural model: application to x-ray powder diffraction data. J. Appl. Cryst., 19: 440-447.
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