Indian Journal of Drugs and Diseases

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Effect of Selected Yoruba Medicinal Formulations on Certain Biochemical Parameters in Wistar Albino Rats


Affiliations
1 Department of Biochemistry, University of Port-Harcourt, Choba, Rivers state, Nigeria
 

Yoruba medicinal formulations derived from plant extracts are fast becoming one of the most widely consumed medicinal formulation in the rural areas of Nigeria. The aim of this study was to screen evaluate the effect of this medicinal formulation on cetain biochemical parameters of wistar albino rats. Sixty-four rats irrespective of sex were used in this study. They were divided into six groups (control and Groups A-E); with groups A-E split into three groups for testing at week 2, 4 and 6. Each sub group contained four rats and was administered 2.9μl/Kg B.W of each medicinal formulation. Cage side examination was done daily to observe overt signs of toxicity (salivation, lacrimation, yellowing of fur etc). The formulation resulted in a significant increase then reduction in the body weight. Markers of hepatocyte injury; alanine transaminase and aspartate transaminase, were initially reduced but increased at week 4. Same was observed for the cholestasis marker alkaline phosphatase. The levels of serum creatinine, urea and blood urea nitrogen steadily reduced over time. The histological findings of the liver sections indicated gross cirrhosis, degeneration, inflammation and apoptosis in the experimented groups as compared to the control group.

Keywords

Yoruba, Biochemical Parameters
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  • Abolaji OA, Adebayo AH, Odesanmi OS (2007) Nutritional Qualities of Three

  • Medicinal Plant Parts (Xylopia aethiopica, Blighia sapida and Parinari polyandra)

  • commonly used by Pregnant Women in the Western Part of Nigeria. Pak. J. Nutr. 6,665-668

  • Adebayo AH, Aliyu R., Gatsing D and Garba IH (2006) The Effects of Ethanolic Leaf

  • Extract of Commiphora africana (Burseraceae) on Lipid Profile in Rats. Int. J.Pharmacol. 2, 618-622.

  • Chevellier A (1996) The Encylopedia of Medical Plant. London.Dorling Kindersley Ltd. (online), Http://www.chclibrary.org/plant.html

  • David LW and Vincent M (1985) Biochemistry in clinical practice. New York: Worth Publishers; Test indicating hepatocellular damage. pp: 142

  • De Smet PAGM (1995) Health risk of Herbal Remedies. Drug Safety.13(2), 81-93

  • Kadiri AB (2008) Evaluation of medicinal herbal trade (Paraga) in Lagos state of Nigeria. Enthnobotanical leaflets 12, 677-681

  • Mac (2002) Safety of herbal medicinal products. www.mhra.gov.uk/home/idcplg?Idcservice

  • Murray RK (2000) Red & White Blood Cells. In: Harpers’s Biochemistry (Granner RK, Mayes PA and Rodwell VW, eds.) Mcgraw-Hill, USA. pp:780-786

  • Nerbert WT (1983) Fundamental of clinical chemistry. In: Burtis CA, Ashwood ER, editors. Tietz Texbook of Clinical Chemistry. 3rd ed. Philadelphia: Saunders WB: pp: 1056–1092

  • Nkosi CZ, Opoku AR and Terblanche SE (2005) Effect of pumpkin seed (Cucurbita pepo) protein isolate on the activity levels of certain plasma enzymes in ccl4 induced liver injury in low protein fed rats. Phytother. Res.19,341–345

  • Olowokudejo JD, Kadiri AB, Travih VA (2008) An ethnobotanical survey of herbal markets and medicinal plants in Lagos state of Nigeria. Ethnobotanical leaflets 12,851-865

  • Olson H, Betton G, Robinson D, Thomas K, Monro A, Kolaja G, Lil YP, Sanders J, Sipes G, Bracken W, Dorato M, Deun KV, Smith P, Berger B, Heller A (2000) Concordance of toxicity of pharmaceuticals in humans and in animals. Regul. Toxicol. Pharmacol. 32, 56-67

  • Shah M, Patel P, Phadke M, Menon S, Francis M and Sane RT (2002) Evaluation of the effect of aqueous extract from powder of ischolar_main, stem, leaves and whole plant of Phyllanthus debilis against CCl4 induced rat liver dysfunction. Indian Drug. 39,333-337

  • Taylor JLS, Rabe T, Mcgaw LJ, Jäger AK and Van Staden J (2001) Towards thescientific validation of traditional medicinal plants. Plant Growth Regul. 34, 23-37

  • Adebayo AH, Tan NH, Akindahunsi AA, Zeng GZ, Zhang YM (2010) Anticancer and antiradical scavenging activity of Ageratum Conyzoides L. (Asteraceae). Pharmacog. Mag. 6, 62-66

  • Yakubu MT, Akanji MA and Oladiji AT (2007) Haematological evaluation in male albino rats following chronic administration of aqueous extract of Fadogia agrestis stem. Pharmacog. Mag. 3,34

  • Yakubu MT, Akanji MA and Oladiji AT (2008) Alterations in serum lipid profile of male rats by oral administration of aqueous extract of Fadogia argrestis stem. Res. J. Med. Plant. 2, 66-73


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  • Effect of Selected Yoruba Medicinal Formulations on Certain Biochemical Parameters in Wistar Albino Rats

Abstract Views: 568  |  PDF Views: 416

Authors

D. C. Belonwu
Department of Biochemistry, University of Port-Harcourt, Choba, Rivers state, Nigeria
E. N. Onyieke
Department of Biochemistry, University of Port-Harcourt, Choba, Rivers state, Nigeria
Oghenekaro Uche Ekere
Department of Biochemistry, University of Port-Harcourt, Choba, Rivers state, Nigeria

Abstract


Yoruba medicinal formulations derived from plant extracts are fast becoming one of the most widely consumed medicinal formulation in the rural areas of Nigeria. The aim of this study was to screen evaluate the effect of this medicinal formulation on cetain biochemical parameters of wistar albino rats. Sixty-four rats irrespective of sex were used in this study. They were divided into six groups (control and Groups A-E); with groups A-E split into three groups for testing at week 2, 4 and 6. Each sub group contained four rats and was administered 2.9μl/Kg B.W of each medicinal formulation. Cage side examination was done daily to observe overt signs of toxicity (salivation, lacrimation, yellowing of fur etc). The formulation resulted in a significant increase then reduction in the body weight. Markers of hepatocyte injury; alanine transaminase and aspartate transaminase, were initially reduced but increased at week 4. Same was observed for the cholestasis marker alkaline phosphatase. The levels of serum creatinine, urea and blood urea nitrogen steadily reduced over time. The histological findings of the liver sections indicated gross cirrhosis, degeneration, inflammation and apoptosis in the experimented groups as compared to the control group.

Keywords


Yoruba, Biochemical Parameters

References