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Study of In-vitro Micropropagation of a Medicinally Important Plant Adhatoda vasica Nees by Auxillary-bud Culture


Affiliations
1 Department of Botany and Biotechnology, Laboratory of Plant Tissue Culture, Government E. Raghvendra Rao P. G. College of Science, Bilaspur - 495 006, Chhattisgarh, India
2 Government Nagarjuna, P. G. College of science, Raipur - 492 010, Chhattisgarh, India
 

The experiment was carried out at plant tissue culture laboratory, Department of Biotechnology, E.R.R.Govt Sci. Auto. P.G. College, Bilaspur, Chhattisgarh, during the period of Nov.08 2010 to May 09 2011. An efficient protocol was devised for rapid in vitro propagation through shoot bud culture of Adhatoda vasica. In present investigation the proliferating auxiliary shoot cultures were established on MS medium&Gamborg B5 medium supplemented with different concentrations of BAP, NAA, Kinetin&2, 4-D using nodal explants from the field grown mature healthy plant of Adhatoda vasica after 30 days of inoculation. In cultures raised from nodal explants of Adhatoda vasica maximum number of shoots was produced on MS medium supplemented with 1.0mg\l BAP. These explants developed greater than two shoots per nodes while in all other concentration of kn, NAA&2,4-D developed either two or less than two shoots\explants. Highest frequencies of shoot formation and maximum number of shoots per explants were obtained on MS medium supplemented with 1mg\l IBA.

Keywords

Adhatoda vasica, Micropropagation, MS Medium, Nodal Explants, Shoot Bud Culture
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  • Aasim M, Khawar KM and Özcan S (2008) In Vitro micro Propagation from shoot meristems of Turkish Cowpea (Vigna unguiculata L.) cultivar Akkız. Bangladesh J. Bot., 37, 149-154.

  • Bjaj M and Williams JT (1995) Healing Forests-Healing People (report on workshop on medicinal plants, 6-8 Feb., 1995, Calicut), IDRC, New Delhi, pp: 62.

  • Che P, Gingerich DJ, Lall S and Howell SH (2002) Global and hormone induced gene expression changes during shoot development in Arabidopsis. Plant cell, 14, 2771-2785.

  • Chopra RN (1982) Indigenous Drugs of India, Academic Publishers, Calcutta.

  • Dastur JF (1985) Medicinal plants of India and Pakistan, DB Taraporevala Sons and co. pvt. Ltd., Bombay.

  • Doshi JJ, Patel VK and Bhatt HV (1983) Effect of Adhatoda vasica massage in pyorrhea. Int. J. Crude Drug Res., 21, 173-176.

  • Kirtikar KR and Basu BD (1994) Adhatoda Vasica Nees, India, 1899 -1902.

  • Murashige T and Skoog F (1962) A revised medium for rapid growth and bioassays for tobacco tissue cultures, Physiol. Plant, 15, 431-491.

  • Osman MG, Elhadi EA and Khalafalla MM (2010) Callus formation and organogenesis of tomato (Lycopersicon esculentum Mill, C.V. Omdurman) induced by thidiazuron. Afr. J. Biotechnol., 9, 4407-4413.

  • Pandey R and Mishra A (2010) Antibacterial activities of crude extract of Aloe barbadensis to clinically isolated bacterial pathogens. Applied biochem. Biotechnol., 160, 1356-1361.

  • Prajapati ND, Purohit SS, Sharma DD and Tarun K (2003) A Handbook of Medicinal Pants. 1st Edn., agrobiaos, Jodhpur, India, pp. 13-14.

  • Prasad PJN, Chakradhar T and Pullaih T (2004) Micropropagation of Cryptolepsis buchanani roem. and schutt. Taiwania, 49, 57-65.

  • Sugiyama M and Imamura K (2006) Dose time and tissue dependent effects of 5-bromo-2’-deoxyuridine on the in vitro organogenesis of Arabidopsis thaliana. Plant Cell Tissue Organ Culture, 87, 17-25.


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  • Study of In-vitro Micropropagation of a Medicinally Important Plant Adhatoda vasica Nees by Auxillary-bud Culture

Abstract Views: 375  |  PDF Views: 74

Authors

Prerna Soni
Department of Botany and Biotechnology, Laboratory of Plant Tissue Culture, Government E. Raghvendra Rao P. G. College of Science, Bilaspur - 495 006, Chhattisgarh, India
A. N. Bahadur
Government Nagarjuna, P. G. College of science, Raipur - 492 010, Chhattisgarh, India
V. K. Kanungo
Government Nagarjuna, P. G. College of science, Raipur - 492 010, Chhattisgarh, India

Abstract


The experiment was carried out at plant tissue culture laboratory, Department of Biotechnology, E.R.R.Govt Sci. Auto. P.G. College, Bilaspur, Chhattisgarh, during the period of Nov.08 2010 to May 09 2011. An efficient protocol was devised for rapid in vitro propagation through shoot bud culture of Adhatoda vasica. In present investigation the proliferating auxiliary shoot cultures were established on MS medium&Gamborg B5 medium supplemented with different concentrations of BAP, NAA, Kinetin&2, 4-D using nodal explants from the field grown mature healthy plant of Adhatoda vasica after 30 days of inoculation. In cultures raised from nodal explants of Adhatoda vasica maximum number of shoots was produced on MS medium supplemented with 1.0mg\l BAP. These explants developed greater than two shoots per nodes while in all other concentration of kn, NAA&2,4-D developed either two or less than two shoots\explants. Highest frequencies of shoot formation and maximum number of shoots per explants were obtained on MS medium supplemented with 1mg\l IBA.

Keywords


Adhatoda vasica, Micropropagation, MS Medium, Nodal Explants, Shoot Bud Culture

References