Indian Journal of Innovations and Developments

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The experiment was carried out at plant tissue culture laboratory, Department of Biotechnology, E.R.R.Govt Sci. Auto. P.G. College, Bilaspur, Chhattisgarh, during the period of Nov.08 2010 to May 09 2011. An efficient protocol was devised for rapid in vitro propagation through shoot bud culture of Adhatoda vasica. In present investigation the proliferating auxiliary shoot cultures were established on MS medium&Gamborg B5 medium supplemented with different concentrations of BAP, NAA, Kinetin&2, 4-D using nodal explants from the field grown mature healthy plant of Adhatoda vasica after 30 days of inoculation. In cultures raised from nodal explants of Adhatoda vasica maximum number of shoots was produced on MS medium supplemented with 1.0mg\l BAP. These explants developed greater than two shoots per nodes while in all other concentration of kn, NAA&2,4-D developed either two or less than two shoots\explants. Highest frequencies of shoot formation and maximum number of shoots per explants were obtained on MS medium supplemented with 1mg\l IBA.

Keywords

Adhatoda vasica, Micropropagation, MS Medium, Nodal Explants, Shoot Bud Culture
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