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Genetic Fidelity Testing in Micropropagated Plantlets of Albizia procera (Roxb.) Using Rapd and Issr Markers
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Random amplified polymorphic DNA (RAPD) and Inter simple sequence repeat (ISSR) markers were employed to check the genetic fidelity of Albizia procera plantlets multiplied in vitro by using nodal explant. A total of 33 distinct and scorable bands were produced by five RAPD primers with an average of 6.6 bands per primer while the number of bands for ISSR primers varied from two (UBC-840) to six (UBC-812), with an average of four bands per primer. All bands were monomorphic across all the micropropagated plants studied. Amplification of monomorphic bands with both marker systems authenticated the true to type nature of the in vitro raised plantlets of Albizia procera. These results suggest that the micropropagation protocol followed in this study is appropriate and applicable for mass clonal propagation of true-to-type superior Albizia procera trees.
Keywords
Molecular Markers, Genetic Fidelity, Somaclonal Variation, Albizia procera.
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