Standardization of in-vivo Nitrate Reductase Activity in Crotalaria burhia Leaves

Combinations of different concentration of substrate (0.10M, 0.15M, 0.20 and 0.25M KNO₃) with different pH of buffer (0.2M, KH₂PO₄ of the pH 6.5, 7.0, 7.5, 7.6, 7.7, 7.8, 7.9, 8.0, 8.1 and 8.2) solution were tried for the nitrate reductase activity of Crotalaria burhia leaves. Maximum nitrate reductase activity was observed in the combination of buffer solution of 0.2M having pH 7.5 and substrate solution of the concentration 0.15M.