Tissue Culture of 40 Year Old Clump of Bambusa Nutans Munro Culture

Nodal segments taken from 40 yr old clump were surface sterilized using 0.1% (w/v) HgCl₂ solution for 15-20 minutes and placed aseptically on media supplemented with 7.5µM BAP for bud break (79.16 ± 7.22) and it was observed within a period of two weeks. Sprouted shoots were excised along with small piece of mother tissue and kept on medium supplemented with 5.0µM BAP for multiplication for 4-5 subculture cycles. Bunch of three shoots exhibited faster shoot multiplication when cultured on multiplication media. During multiplication phase, BAP alone proved to be superior to kinetin. Microshoots exhibited higher ischolar_maining percentages on media supplemented with IBA than other auxins (IAA or NAA). Effective IBA concentration was found to be 10µM on which 72.22% of propagules ischolar_mained. Hardening was effectively carried out on half strength MS media without growth regulators, vitamins and sucrose. Success was achieved in acclimatizing 73.33% ischolar_mained propagules on mixture carrying potting mix of sand:FYM: Soil in l:l:l in poly-tunnels. Acclimatized plantlets were successfully transplanted to pots.