Indian Forester

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Evaluation of Four Different Methods of Genomic DNA Extraction from Quercus leucotricophora


Affiliations
1 Division of Genetics and Tree Propogation, Forest Research Institute University, Dehradun-243195, India
     

   Subscribe/Renew Journal


The most essential principal in the modern molecular biology is extraction of DNA with a desirable quantity and quality and this achievement could be the main necessity for every genetic study. DNA extraction is obviously difficult because of negative effects obtained from carbohydrates, tannins, polyphenols and proteins. Extraction of DNA from Quercus leucotricophora, was conducted by four different methods. The most value of qualified DNA extracted from young leaf tissue was 629.9 to 1412.1 ng/μl by the method applied by Doyle and Doyle and Stange and his associates with some modifications. Accordingly above method is recommended for DNA extraction from young leaves of Quercus leucotricophora.

Keywords

DNA, Extraction, Quercus leucotricophora.
Font Size

User
About The Authors

Sneha Dobhal
Division of Genetics and Tree Propogation, Forest Research Institute University, Dehradun-243195
India

H. S. Ginwal
Division of Genetics and Tree Propogation, Forest Research Institute University, Dehradun-243195
India


Subscription Login to verify subscription
Notifications

  • Bradshaw, H.D. and Stetler, R.F. (1993). Molecular genetics of growth and development in Populus.I. Triploidy in hybrid Poplars. Theor. App.Genet., 86: 301-307.

  • Bushra, C.; Afshan, Y.; Tayyab,H. and Riazuddin, S. (1999). Mini-scale genomic DNA extraction from cotton. Plant Mol. Biol. Rep., 17: 1–7.

  • Doyle, J.J. and Doyle, J.L. (1990). Isolation of plant DNA from fresh tissue. Focus, 12: 11–5.

  • Laura, M.; Benedetti, L. De.; Bruna, S.; Burchi, G.; Berio, T.; Giovannini, A. and Ruffoni, B.(2003). Cyclamen persicum mill: Somatic embryogenesis and RAPD analysis of embryogenic callus. Acta Hort., (ISHS) 625: 137–44

  • Lodhi, M.A.; Ye, G.N.; Weeden, N.F. and Reisch, B.I. (1998). A simple and efficient method DNA extraction from grapevine caltivars and vitis species. Plant Mol. Biol. Rep., 12: 6–13.

  • Naderi, R.; Khalighi, A.; Arzani, K. and Babalar, M. (2000). Effects of storage temperature, chemical treatment, harvesting stage and genotype on vase life of cut flowers of cyclamen (Cyclamen persicum Mill.). Seedand Pl. J., (SPII), 16: 240–51

  • Porebski, S.; Bailey L. and Boum; B.R. (1997). Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components. Plant Mol. Biol. Rep., 15: 8–15.

  • Stange, C.; Prehn, D. and Johnson, P.A. (1998). Isolation of Pinus radiata genomic DNA suitable for RAPD analysis. Plant Molecular Biology Reporter, 16:1-8.

  • Zhang, J.; McDonald, M.B. and Sweeney, P.M. (1997). Testing for genetic purity Petunia and Cyclamen seed using random amplified polymorphic DNA markers. J. Amer. Soc. Hort. Sci., 32: 246–7.


Abstract Views: 217

PDF Views: 0




  • Evaluation of Four Different Methods of Genomic DNA Extraction from Quercus leucotricophora

Abstract Views: 217  |  PDF Views: 0

Authors

Sneha Dobhal
Division of Genetics and Tree Propogation, Forest Research Institute University, Dehradun-243195, India
H. S. Ginwal
Division of Genetics and Tree Propogation, Forest Research Institute University, Dehradun-243195, India

Abstract


The most essential principal in the modern molecular biology is extraction of DNA with a desirable quantity and quality and this achievement could be the main necessity for every genetic study. DNA extraction is obviously difficult because of negative effects obtained from carbohydrates, tannins, polyphenols and proteins. Extraction of DNA from Quercus leucotricophora, was conducted by four different methods. The most value of qualified DNA extracted from young leaf tissue was 629.9 to 1412.1 ng/μl by the method applied by Doyle and Doyle and Stange and his associates with some modifications. Accordingly above method is recommended for DNA extraction from young leaves of Quercus leucotricophora.

Keywords


DNA, Extraction, Quercus leucotricophora.

References