In Vitro Propagation of Elaeocarpus Sphaericus

The present study involves in-vitro clonal propagation of Elaeocarpus sphaericus through axillary bud sprouting and subsequent shoot regeneration. Optimal proliferations of axillary shoot were induced on MS medium supplemented with BAP (1.5 mg/l) and KN (1.5 mg/l). Optimal shoot multiplication was induced on MS medium fortified with BAP (1.5mg/l), KN (1.5 mg/l) and CH (100 mg/l). MS medium supplemented with 1.0 mg/l BAP and 50 mg/l ADS was used for routine sub-culturing of multiple shoots at periodic interval of every 3 weeks. Of the 6 treatments (3 each of IBA and NAA) imposed for ischolar_maining of shoots, 1/2 MS + 1.0 mg/l IBA induced 80% ischolar_maining. Plants were hardened in vitro and acclimatized in hardening chamber and subsequently transferred to field.