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Study of Prognostic Factors affecting on the IUI Outcome of Infertile Patients


Affiliations
1 University of Kufa, Faculty of Science Department of Biology, Najaf, Iraq
2 Department of Drugs and Toxins – College of Pharmacy–Kufa University, Iraq
     

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Objective: The main objective of the study is to determine of prognostic factors on the pregnancy rate after intrauterine insemination (IUI) of infertile patients. Methods: The study was carried out between January 2014 and October 2014 including one hundred and fifty five infertile men who attended the Fertility Center in Al – Sadr Medical City. Results: The of the study showed that there was statistically significant (p<0.01) increase in pregnancy rate with couples that had sperm concentration ≥20 × 106/ml, progressive motility (A +B) ≥ 50 % and normal morphology ≥ 30% as well as couples with leukocyte concentration < 1×106 /ml, MDA concentration < 4 μmol/L and sperm chromatin maturity < 30%. This results also appeared higher significant (p<0.01) with couples that had age <30 years, infertility duration < 6 years and with couples non – smokers compared to smokers. While no significant difference (p> 0.01) was showed with type of infertility. Conclusion: Many factors may influence success rate after IUI treatment procedures such as potential sperm parameters, leukocyte concentration, MDA concentration and sperm chromatin maturity as well as age, infertility duration and smoking. Recommendation: Advance study to determine of the effect female factors on the IUI outcome.

Keywords

Infertility, IUI, Sperm Parameters, Leukocyte Concentration, Sperm Chromatin Maturity.
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  • Alborzi, s., Motazedian, parsanezhud, ME, jannati, S: comparison of the effectiveness of single IUI versus double IUI per cycle in infertile patients.fertil steril. 2003; 80 :595 – 599.

  • Omblete, W., Compo, R., Bosmans, E and Nijs, M.: Intrauterine insemination (IUI) as a first – line treatment in developing countries and methodology cal aspect that night influence IUI success. Hum Reprod. 2008; 23 (1): 64 – 72.

  • Zayed, F., lent on, E.A and cooke, ID.: Comparison between stimulated in – vitro fertilization and stimulated intra uterine in semi nation for the treatment of unexplained and mild male factor infertility. Hum reprod. 1997 ; 12: 2408 – 2413.

  • Huang, H Y., Lee, CI., Lai, YM., chang, MY., Wang, Hsand chang, SY.: The impact of the total motile sperm count on the success of intrauterine insemination with husbands spermatozoa. J Assist reprod Genet. 1996; 13: 56 -63.

  • Burr, RW., Siegberg, R., Flaherty, sp., Wang, XJ and Matthews, CD.: The influence of sperm morphology and the number of motile sperm inseminated on the outcome of motile sperm insemination combined with mild ovarian stimulation. Fertile steril. 1996; 65: 127 – 132.

  • 6-Morrell, JM., Moffatt, O., Sakkas, D., Manicardi, GC., Bizzaro, Dand Tomlinson, M.: Reduced senescence and retained nuclear DWA integrity in human spermatozoa prepared by density gradient centrifugation. J Assist Reprod Gerent. 2004; 21: 217 222.

  • Word health organization (WHO): WHO laboratory manual for the examination of human semen and semen – cervical mucus interaction. Ed. New York. Cambridge University Press UK. 1999; 4 -59.

  • Zafar, M., Jameel, T and Abdulloh, KW.: Impact of intrauterine insemination as first line treatment of subertility. J pak Med Assoc. 2007; 57 (3): 133 – 136.

  • Wainer, R., Albart, M., Dorion, A., Bailly, M., Bergere, Mandlombroso, Retal: Influence of the number of motile spermatozoa inseminated and of their morphology on the success of intrauterine insemination. Hum Reprod.2004 ; 19:2060-2065.

  • Marshburn, PB., Melntire, D., Carr, BP. And Byrd, W.: Spermatozoal characteristics from fresh and frozen fertility outcome after intrauterine insemination. Fertile Sterile. 1992; 58:179-86.

  • Aitken , RJ., Best, FS. And Richardson , DW, et al.:Ananalysis of sperm function incases of unexplained infertility: conventional criteria, movement characteristics , and fertilizing capacity. Fertil Steril. 1982; 38:212-21.

  • Speroff, L and Fritz, MA.: Male infertility. In : clinical Gynecologic Endocainnology and infertility. Baltimore, Williams and Wilkins. 2005; 30:1135-1173.

  • Merriel, P., Heraud, MH., Grenier, N., Lourdel, E., Sanguinet, P and Copin, H: Predictive factors for pregnancy afer intrauterine insemination (IUI): an analysis of 1038 cycles and a review of the Literature. Fertil Steril.2010; 93:79-88.

  • Lee, RK., Hou, JW., HO, Hy., Hwu, YM., Lin, MH and Tsai, YC et al.: sperm morphology analysis using strict criteria as aprognostic factor in intrauterine insemination. Int J Androl. 2002; 25:277.

  • Henkel, R., Kierspel, E and Stalf, T.: Effect of reactive Oxygen species produced by spermatozoa and Leukocytes on sperm functions in non-Leukocytospermic patients. Fertil steril. 2005; 83 : 635 – 642.

  • Erenpreiss, J., Hlevicka, S. and Zalkalns, J.: Effect of leukocyte spermia on sperm DWA integrity: anegative effect in abnormal semen samples. J Androl. 2002; 23: 717 – 723.

  • Giwercman, A., Lindstedt, l., larsson, M., Bungum, M., Spano, M and Levine, R.J: Sperm chromatin structure assay as an independent predictor of fertility in vivo: a case –control study. Int J Androl. 2010; 33: 221 - 227.

  • Loft, S. Kold – Jensen, T., Hjollund, N.H., Giwercman, A., Gyllemborg,, J and Ernst, E.: Oxidative DWA damage in human sperm influences time to pregnancy. Hum Reprod. 2003; 18: 1265 – 1572.

  • Evenson, D. Wixon, R.: Meta-analysis of sperm DWA fragmentation using the sperm chromatin structureassay. Reprod. Biomed. Online. 2006; 12(4): 466 - 72.

  • Evenson, DP., Larson, KL and Jost, LK.: Sperm chromatin strunture assay: its clinical use for detecting sperm DWA fragmentation in male infertility and comparisons with other techniques. J. Androl. 2002; 23(1):25-43.

  • Zini, A and Sigman, M.:Are tests of sperm DWA damage clinically useful? Pros and cons. J. Androl. 2009; 30(3): 219 – 29 22. Oral Ozay., Kutlu Tayfun., Aksoy Evrim., Ficicioglu cem., Uslu Husamettin and of oxidative stress on outcomes of Assisted reproductive Techingues. J Assist Reprod Genet; 23 (2): 81 – 85.

  • Hsieh, YY., chang, C and lin, CS.: seminal malondialdehyde concentration but not glutathione peroxidase activity is negatively correlated with seminal concentration and motility. Int. J. Biol. Sci. 2006; 2(1):23.

  • Aitken, RJ and krausz,C.: Oxidative stress, DWA damage and the Y chromosome. Reproduction.1999; 122: 497 – 506.

  • Shen, HM., Chia, SE. and ong, CW.: Evuluation of oxidative DWA damage in human sperm and its association with male infertility. J Androl. 2000; 20: 718 – 23.

  • Jason, R., Josephine, A., Ryan, p., Robert, M., Dolores, J and Larry, I.: The effects of advanced paternal ago on fertility. Asian Journal of Andrology. 2013; 15:723 -728.

  • Luna, M., Finkler, E., Barritt, J., Bar-Chama, N. and Sandler, Betal.: paternal ago and assisted reproductive technology outcome in ovum recipients. Fertil steril. 2009; 92:1772-5.

  • dela Rochebrochard, E and Thonneau, P.: paternal ago ≥ 40 years: an important risk factor for infertility. Am J. Obstet Gyecol; 189:901- 5.

  • Olsen, J (1990) Sub fecundity according to the ago of the mother and the father. Dan Med Bull. 2003; 34:281-2.

  • Snick, HK., Snick, Ts., Evers, JL and Collins, JA.: The spontaneous pregnancy prognosis in untreated sub fertile couples: the Walcheren primary care study. Hum reprod. 1997;12: 1582 8.

  • Tomlinson, M., Amissah – Arthur J., Thompson, K., Kasraie, J and Bentick, B.: Infertility: prognostic indicators for intrauterine insemination (IUI): Statistical model for IUI success. Hum Reprod. 1996; 11: 1892 – 6.

  • Zahra Basirat and Seddigheh Esmaeilzadeh: prognostic factors of pregnancy in 500 cases of intrauterine insemination in Babol, northern Iran. Royal Institute international Journal of Fertil and steril. 2010; 4(1):35-39.

  • Alvarez, J,G.: DNA Fragmentation in human spermatozoa significance in the diagnosis and treatment of infertility. Minerva Ginecol. 2003; 55: 233 – 239.

  • potts, RJ., Newbury, CJ., smith, G., Notarianni, LJ and Jefferies, TM.: Sperm chromation damage associated with male smoking. Mutat Res. 1999; 423:103- 111.

  • Saleh, R.,Agarwal, A., Sharma, R., Nelson, D. and Tomas, A.J.: Effect of cigarette smoking on levels of seminal oxidative stress in infertile men: a prospective study. Fertile steril. 2002; 78: 491 – 499.


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  • Study of Prognostic Factors affecting on the IUI Outcome of Infertile Patients

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Authors

Mohsin K. Al- Murshdi
University of Kufa, Faculty of Science Department of Biology, Najaf, Iraq
Yahya K. Al – Sultani
Department of Drugs and Toxins – College of Pharmacy–Kufa University, Iraq

Abstract


Objective: The main objective of the study is to determine of prognostic factors on the pregnancy rate after intrauterine insemination (IUI) of infertile patients. Methods: The study was carried out between January 2014 and October 2014 including one hundred and fifty five infertile men who attended the Fertility Center in Al – Sadr Medical City. Results: The of the study showed that there was statistically significant (p<0.01) increase in pregnancy rate with couples that had sperm concentration ≥20 × 106/ml, progressive motility (A +B) ≥ 50 % and normal morphology ≥ 30% as well as couples with leukocyte concentration < 1×106 /ml, MDA concentration < 4 μmol/L and sperm chromatin maturity < 30%. This results also appeared higher significant (p<0.01) with couples that had age <30 years, infertility duration < 6 years and with couples non – smokers compared to smokers. While no significant difference (p> 0.01) was showed with type of infertility. Conclusion: Many factors may influence success rate after IUI treatment procedures such as potential sperm parameters, leukocyte concentration, MDA concentration and sperm chromatin maturity as well as age, infertility duration and smoking. Recommendation: Advance study to determine of the effect female factors on the IUI outcome.

Keywords


Infertility, IUI, Sperm Parameters, Leukocyte Concentration, Sperm Chromatin Maturity.

References