Research Journal of Pharmacy and Technology

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Isolation and Characterization of Phytochemical Constituents and its Antibacterial Activity of Brassica oleracea var acephala


Affiliations
1 Universiti Sultan Zainal Abidin, 20400 Terengganu, Malaysia
2 PG & Research Department of Microbiology, Hindustan College of Arts & Science, Padur, Chennai–603103, India
3 PG & Research Department of Biotechnology, Hindustan College of Arts & Science, Padur, Chennai–603103, India
     

   Subscribe/Renew Journal


Objectives: Traditional medicines are mostly depends on medicinal plants. These are considered as good resources of ingredients which can be used in drug formulation and combination. Hence the present study is to investigate the valuable pharmacological activities ofBrassica oleracea var acephala. Materials and Methods: The collected fresh foliage were washed, dried, chopped and powdered for the preparation of ethanolic crude extract. By using this ethanolic crude extract of Brassica oleracea var acephala phytochemical analysis were done. The crude extract was tested against various bacterial strains and fungal strains by Agar Well diffusion assay. Then the crude extract was subjected to GC-MS and FTIR analysis for the identification of bioactive compounds. Results: Preliminary phytochemical test showed the presence of Phenolic compounds, Tannins, Terpenoids, Phytosterol and Flavonoids.The results of the antibacterial screening of the crude extract on the S.aureus showed more inhibition. The ethanolic crude extract showed that eleven compounds were identified through GC-MS and FTIR analysis. Conclusion: Brassica oleracea var acephala has been studied and the results of the present investigation revealed that the leaf extract contained high phytochemical activity. The present study attempted to establish a relationship between ethnopharmacological claims and bioactive constituents present in Brassica oleracea var Acephala against all possible targets for cancer.

Keywords

Brassica oleracea var acephala, Phytochemical Screening, Antibacterialactivities, GC-MS Analysis, FTIR Analysis.
Subscription Login to verify subscription
User
Notifications
Font Size


  • Shariff, ZU (2001). Modern Herbal Therapy for Common Ailments. Nature Pharmacy series Vol.1, spectrum Books Ltd., Ibadam, Nigeria in Association with Safari Books Ltd. UK, pp 9-84.

  • Rindos, D. (1984). The Origins of Agriculture: An Evolutionary Perspective. New York, NY:Academic Press.

  • Ramachandran A et al, (2002). Burden of type 2 diabetes and its complications-the Indian scenario. Curr. Sci 83: 1471-1476

  • Fransworth, NR (1992). The Role of Ethano Pharmacology in Drug Development. Ciba Foundation Symposium 154. Bioactive Compounds from Plants. John Wiley and Sons, Baffins Lane, Chichester (England), 2-21.

  • Farnsworth, NR, Soejarto, DD (1990) Global importance of medicinal plants. In: Conservation of Medicinal Plants. Cambridge University Press, UK 25-52.

  • Davidson-Hunt, I (2000). Ecological ethno botany: stumbling toward new practices and paradigms. (MASA) J 16:1–13.

  • Zhao H, Lin J, Grossman HB (2007) Dietary isothiocyanates, GSTM1, GSTT1, NAT2 pol ymorphisms and bladder cancer risk. Int J Cancer 120:2208-13.

  • Van Poppel, G et al.,(1999). Brassica vegetablesand cancer prevention. Epidemiology and mechanisms. AdvExp Med Biol 472:159-68.

  • Pantuck, EJ et al., (1984). Effect of brussels sprouts and cabbage on drug conjugation. Clin PharmacolTher 35:161-9.

  • Platel K, Srinivasan K (1997). Plant foods in the management of diabetes mellitus: vegeta bles as potential hypoglycaemic agents. Nahrung 41 (2): 68-74.

  • Kojima T, Tanaka T, Mori H (1994). Chemoprevention of spontaneous endometrial cancer in female Donryu rats by dietary indole-3-carbinol. Cancer Res 54:1446-9

  • Schmidt S, et al., (2010) Genotypic and climatic influences on the concentration and composition of flavonoids in Kale (Brassica oleracea var. sabellica). Food Chem. 119:1293–9.

  • *Stan N, Munteanu N (2001).Legumiculturăspecială, vol.II. Editura “Ion Ionescu de la Brad” Iaşi.

  • Eberhardt, F (2007). Causation and Intervention. PhD thesis, Carnegie Mellon.

  • Hazra KM, Roy RN, Sen SK, Laska S (2007). Isolation of antibacterial pentahydroxy flavones from the seeds of Mimusopselengi Linn. Afr. J.Biotechnol. 6(12):1446–1449.

  • Ayshwarya M et al., (2015). Qualitative and quantitative analysis ofphytochemicals in Brassica oleraceavarcapitatarubra. Int. J of multidisciplinary research; (4) 6 (4): 120-136.

  • Narendradevanaboyina, N et al., (2013). Preliminary phytochemical screening, quantitative estimation and evaluation of antimicrobial activity of Alstoniamacrophylla stem bark. Int. J. of Sci. Inventions Today, 2, (1).

  • Shinde SA, Kadam SS, Suryawanshi JS, Tare, HL (2013) Antimicrobial activity of Brassica oleracea leaves. Int. J. of universal pharmacy and life sciences 3(2): March-April


Abstract Views: 152

PDF Views: 0




  • Isolation and Characterization of Phytochemical Constituents and its Antibacterial Activity of Brassica oleracea var acephala

Abstract Views: 152  |  PDF Views: 0

Authors

U. S. Mahadeva Rao
Universiti Sultan Zainal Abidin, 20400 Terengganu, Malaysia
C. Shanmuga Sundaram
PG & Research Department of Microbiology, Hindustan College of Arts & Science, Padur, Chennai–603103, India
J. Sivakumar
PG & Research Department of Biotechnology, Hindustan College of Arts & Science, Padur, Chennai–603103, India

Abstract


Objectives: Traditional medicines are mostly depends on medicinal plants. These are considered as good resources of ingredients which can be used in drug formulation and combination. Hence the present study is to investigate the valuable pharmacological activities ofBrassica oleracea var acephala. Materials and Methods: The collected fresh foliage were washed, dried, chopped and powdered for the preparation of ethanolic crude extract. By using this ethanolic crude extract of Brassica oleracea var acephala phytochemical analysis were done. The crude extract was tested against various bacterial strains and fungal strains by Agar Well diffusion assay. Then the crude extract was subjected to GC-MS and FTIR analysis for the identification of bioactive compounds. Results: Preliminary phytochemical test showed the presence of Phenolic compounds, Tannins, Terpenoids, Phytosterol and Flavonoids.The results of the antibacterial screening of the crude extract on the S.aureus showed more inhibition. The ethanolic crude extract showed that eleven compounds were identified through GC-MS and FTIR analysis. Conclusion: Brassica oleracea var acephala has been studied and the results of the present investigation revealed that the leaf extract contained high phytochemical activity. The present study attempted to establish a relationship between ethnopharmacological claims and bioactive constituents present in Brassica oleracea var Acephala against all possible targets for cancer.

Keywords


Brassica oleracea var acephala, Phytochemical Screening, Antibacterialactivities, GC-MS Analysis, FTIR Analysis.

References