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Isolation and Characterization of Phytochemical Constituents and its Antibacterial Activity of Brassica oleracea var acephala


Affiliations
1 Universiti Sultan Zainal Abidin, 20400 Terengganu, Malaysia
2 PG & Research Department of Microbiology, Hindustan College of Arts & Science, Padur, Chennai–603103, India
3 PG & Research Department of Biotechnology, Hindustan College of Arts & Science, Padur, Chennai–603103, India
     

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Objectives: Traditional medicines are mostly depends on medicinal plants. These are considered as good resources of ingredients which can be used in drug formulation and combination. Hence the present study is to investigate the valuable pharmacological activities ofBrassica oleracea var acephala. Materials and Methods: The collected fresh foliage were washed, dried, chopped and powdered for the preparation of ethanolic crude extract. By using this ethanolic crude extract of Brassica oleracea var acephala phytochemical analysis were done. The crude extract was tested against various bacterial strains and fungal strains by Agar Well diffusion assay. Then the crude extract was subjected to GC-MS and FTIR analysis for the identification of bioactive compounds. Results: Preliminary phytochemical test showed the presence of Phenolic compounds, Tannins, Terpenoids, Phytosterol and Flavonoids.The results of the antibacterial screening of the crude extract on the S.aureus showed more inhibition. The ethanolic crude extract showed that eleven compounds were identified through GC-MS and FTIR analysis. Conclusion: Brassica oleracea var acephala has been studied and the results of the present investigation revealed that the leaf extract contained high phytochemical activity. The present study attempted to establish a relationship between ethnopharmacological claims and bioactive constituents present in Brassica oleracea var Acephala against all possible targets for cancer.

Keywords

Brassica oleracea var acephala, Phytochemical Screening, Antibacterialactivities, GC-MS Analysis, FTIR Analysis.
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  • Isolation and Characterization of Phytochemical Constituents and its Antibacterial Activity of Brassica oleracea var acephala

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Authors

U. S. Mahadeva Rao
Universiti Sultan Zainal Abidin, 20400 Terengganu, Malaysia
C. Shanmuga Sundaram
PG & Research Department of Microbiology, Hindustan College of Arts & Science, Padur, Chennai–603103, India
J. Sivakumar
PG & Research Department of Biotechnology, Hindustan College of Arts & Science, Padur, Chennai–603103, India

Abstract


Objectives: Traditional medicines are mostly depends on medicinal plants. These are considered as good resources of ingredients which can be used in drug formulation and combination. Hence the present study is to investigate the valuable pharmacological activities ofBrassica oleracea var acephala. Materials and Methods: The collected fresh foliage were washed, dried, chopped and powdered for the preparation of ethanolic crude extract. By using this ethanolic crude extract of Brassica oleracea var acephala phytochemical analysis were done. The crude extract was tested against various bacterial strains and fungal strains by Agar Well diffusion assay. Then the crude extract was subjected to GC-MS and FTIR analysis for the identification of bioactive compounds. Results: Preliminary phytochemical test showed the presence of Phenolic compounds, Tannins, Terpenoids, Phytosterol and Flavonoids.The results of the antibacterial screening of the crude extract on the S.aureus showed more inhibition. The ethanolic crude extract showed that eleven compounds were identified through GC-MS and FTIR analysis. Conclusion: Brassica oleracea var acephala has been studied and the results of the present investigation revealed that the leaf extract contained high phytochemical activity. The present study attempted to establish a relationship between ethnopharmacological claims and bioactive constituents present in Brassica oleracea var Acephala against all possible targets for cancer.

Keywords


Brassica oleracea var acephala, Phytochemical Screening, Antibacterialactivities, GC-MS Analysis, FTIR Analysis.

References