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In-Silico and in-Vitro Evaluation of Xanthine Oxidase Inhibition of Zingiber officinalae for Hypouricemic Activity


Affiliations
1 Department of Pharmacology, Sri Ramachandra Institute of Higher Education and Research (Deemed to be University), Chennai, Tamil Nadu, India
2 Department of Pharmacology, Sri Ramachandra Institute of Higher Education and Research (Deemed to be University) Chennai, India
     

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Zingiber officinale (ZO), traditionally known for its remedial potential, was examined on phytoconstituents analysis, antioxidant and xanthine oxidase inhibition by in-vitro analysis, followed by an in-silico study to identify the biological rationale. Results show that ethanolic extract of Zingiber officinale illustrated potential antioxidant to that of standard. DPPH ZO (IC50=42.09ug/mL), DPPH butylhydroxyltoluene (IC50=66.67ug/mL), nitric oxide ZO (IC50=28.36ug/mL), nitric oxide ascorbic acid (IC50=42.87𝜇g/mL). Zingiber officinale extract inhibited the xanthine oxidase activity (IC50 = 188.5ug/mL) compared to allopurinol (IC50=499.2ug/mL), which showed competitive inhibition. The in-silico docking studies of the major phytoconstituents revealed by GC-MS analysis from this extract suggest that the inhibitory activity may be due to the combined effect of these compounds. These results suggest substantial reason for the evaluation of active compounds in in-vivo system for the management and treatment of gout as well as in the oxidative stress conditions.

Keywords

Zingiber officinale, Anti-Oxidant assays, Molecular Docking, Hypouricemic.
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  • In-Silico and in-Vitro Evaluation of Xanthine Oxidase Inhibition of Zingiber officinalae for Hypouricemic Activity

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Authors

Sivakumar Muthusamy
Department of Pharmacology, Sri Ramachandra Institute of Higher Education and Research (Deemed to be University), Chennai, Tamil Nadu, India
Srikanth Jeyabalan
Department of Pharmacology, Sri Ramachandra Institute of Higher Education and Research (Deemed to be University) Chennai, India

Abstract


Zingiber officinale (ZO), traditionally known for its remedial potential, was examined on phytoconstituents analysis, antioxidant and xanthine oxidase inhibition by in-vitro analysis, followed by an in-silico study to identify the biological rationale. Results show that ethanolic extract of Zingiber officinale illustrated potential antioxidant to that of standard. DPPH ZO (IC50=42.09ug/mL), DPPH butylhydroxyltoluene (IC50=66.67ug/mL), nitric oxide ZO (IC50=28.36ug/mL), nitric oxide ascorbic acid (IC50=42.87𝜇g/mL). Zingiber officinale extract inhibited the xanthine oxidase activity (IC50 = 188.5ug/mL) compared to allopurinol (IC50=499.2ug/mL), which showed competitive inhibition. The in-silico docking studies of the major phytoconstituents revealed by GC-MS analysis from this extract suggest that the inhibitory activity may be due to the combined effect of these compounds. These results suggest substantial reason for the evaluation of active compounds in in-vivo system for the management and treatment of gout as well as in the oxidative stress conditions.

Keywords


Zingiber officinale, Anti-Oxidant assays, Molecular Docking, Hypouricemic.

References