Research Journal of Pharmacy and Technology

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Preparation and Physical Characterization of Andrographolide-Chitosan-Alginate Microparticles prepared by Ionic Gelation–Freeze Drying


Affiliations
1 Department of Pharmaceutics, Universitas Airlangga, Dharmawangsa Dalam, Surabaya 60268, Indonesia
     

   Subscribe/Renew Journal


This study aimed to determine the effect of alginate concentration on physical characterization (morphology, physical state, and encapsulation efficiency) of andrographolide-chitosan-alginate microparticles prepared by ionic gelation- freeze drying method. Andrographolide-chitosan-alginate microparticles were produced with various amount of alginate by ionic gelation method using tripholyphospate (TPP) as crosslinker and maltodextrin as a lyoprotectant. The physical characteristics were evaluated using Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectrophotometer (FT-IR), Differential Thermal Analyzer (DTA), Powder X-ray diffractrometer (PXRD), drug content was evaluated using High Pressure Liquid Chromatography (HPLC). The results showed that andrographolide crystallinity changed after encapsulated inside the microparticles supported by changes in IR spectra, and melting point. Furthermore, increased alginate concentration showed rougher surface morphology. The amount of alginate affected the characteristics of the andrographolide-chitosan-alginate microparticles by altering the crystallinity and lowering melting point. The highest encapsulation efficiency shown by microparticles with 0.5% alginate.

Keywords

Microparticles, Andrographolide, Chitosan, Alginates, Ionic Gelation.
Subscription Login to verify subscription
User
Notifications
Font Size


  • Jarukamjorn K, Nemoto N. Pharmacological Aspects of Andrographis paniculata on Health and Its Major Diterpenoid Constituent. 2008;54(4):370-381.

  • VK. BSKVPMG. Validated HPTLC Method for the Quantitation of Andrographolide from Raw Material and Pharmaceutical Dosage Form. Asian J Res Chem. 2009;2(3):314-317.

  • Jadhao M, Wankhade V. Estimation and Quantification of Andrographolide in Herbal Powder and Polyherbal Asava by HPTLC. Asian J Res Chem. 2011;4(1):68-70.

  • Suneetha A, K.Manasa. Development and Validation of Visible Spectrophotometric Method for Determination of Andrographolide in Kalmegh Plant Extract. Asian J Pharm Anal. 2014;4(2):85-88.

  • Chellampillai B, Pandurang A. Improved bioavailability of orally administered andrographolide from pH-sensitive nanoparticles. 2011:123-129. doi:10.1007/s13318-010-0016-7.

  • Xu J, Ma Y, Xie Y, et al. Design and Evaluation of Novel Solid Self-Nanodispersion Delivery System for Andrographolide. AAPS PharmSciTech. 2017;18(5):1572-1584. doi:10.1208/s12249-016-0627-7.

  • Senthil SP, Senthilkumar KL, Chandi SR, Ezhilmuthu RP, Saravanan MM, Sandu NR. Formulation and Evaluation of Imatinib Mesylate Microspheres by Chemical Crosslinking Method. Res J Pharm Technol. 2012;5(7):934-937.

  • Jain V, Garg G, Patil UK, Jain S. Recent Perspectives of Chitosan: A Review. Res J Pharm Dos Forms Technol. 2010;2(3):220-224.

  • Bisen A, Jain AP, Jain S. Formulation and Evaluation of Zidovudine Loaded Microsphere. Asian J Res Pharm Sci. 2013;3(4):200-205.

  • Birnbaum DT, Brannon-Peppas L. Microparticle Drug Delivery Systems. In: Brown DM, ed. Drug Delivery Systems in Cancer Therapy. Totowa, NJ: Humana Press; 2004:117-135. doi:10.1007/978-1-59259-427-6_6.

  • Swarbrick J, ed. Encyclopedia of Pharmaceutical Technology, Third Edition. Third Edit. New York: Informa Healthcare USA, Inc.; 2007.

  • Irianto, Hari E., Muljanah I. Proses dan Aplikasi Nanopartikel Kitosan Sebagai Penghantar Obat. Squalen. 2011;6(1):1-8.

  • Sinha VR, Singla AK, Wadhawan S, et al. Chitosan microspheres as a potential carrier for drugs. Int J Pharm. 2004;274(1-2):1-33. doi:10.1016/j.ijpharm.2003.12.026.

  • Rafeeq M, Junise V, Saraswathi RK, Dilip C. Development and characterization of chitosan nanoparticles loaded with isoniazid for the treatment of Tuberculosis. Res J Pharm Biol Chem Sci. 2010;1(4):383-390.

  • R. Suresh Kumar*, Subhashish Debnath, GNK Ganesh, L Raju MS and BS. Chitosan Nano Particles by Ionotropic Gelation Containing L-Arginine.

  • Rastogi R, Sultana Y, Aqil M, et al. Alginate microspheres of isoniazid for oral sustained drug delivery. Int J Pharm. 2007;334(1):71-77. doi:https://doi.org/10.1016/j.ijpharm.2006.10.024.

  • Tiyaboonchai W. Chitosan Nanoparticles : A Promising System for Drug Delivery. 2003;11(3):51-66.

  • R. Suresh Kumar, Subhashish Debnath, GNK Ganesh, L Raju, MK Samantha BS. Alginate-Based Microparticulate Oral Drug Delivery System for Rifampicin. Res J Pharm Technol. 2009;2(2):301-303.

  • Agnihotri SA, Mallikarjuna NN, Aminabhavi TM. Recent advances on chitosan-based micro- and nanoparticles in drug delivery. J Control Release. 2004;100(1):5-28. doi:10.1016/j.jconrel.2004.08.010.

  • Ko JA, Park HJ, Hwang SJ, Park JB, Lee JS. Preparation and characterization of chitosan microparticles intended for controlled drug delivery. Int J Pharm. 2002;249(1):165-174. doi:https://doi.org/10.1016/S0378-5173(02)00487-8.

  • Debnath S, Kumar RS, Babu MN. Ionotropic Gelation – A Novel Method to Prepare Chitosan Nanoparticles. Res J Pharm Technol. 2011;4(4):492-495. http://anvpublication.org/HTMLPaper.aspx?Journal=Research Journal of Pharmacy and Technology;PID=2011-4-4-1. Accessed October 22, 2018.

  • Sankalia MG, Mashru RC, Sankalia JM, Sutariya VB. Reversed chitosan-alginate polyelectrolyte complex for stability improvement of alpha-amylase: Optimization and physicochemical characterization. Eur J Pharm Biopharm. 2007;65(2):215-232. doi:10.1016/j.ejpb.2006.07.014.

  • Jia-hui Y, Yu-min D, Hua Z. Blend films of chitosan-gelatin. Wuhan Univ J Nat Sci. 1999;4(4):476. doi:10.1007/BF02832288.

  • Manca ML, Zaru M, Ennas G, et al. Diclofenac-beta-cyclodextrin binary systems: physicochemical characterization and in vitro dissolution and diffusion studies. AAPS PharmSciTech. 2005;6(3):E464-E472. doi:10.1208/pt060358.

  • Setyawan D, Sumirtapura YC, Soewandhi SN, Daryono D, Tj H. Karakterisasi sifat fisik sistem biner eritromisin stearat-sodium starch glycolate akibat pengaruh tekanan kompresi Characterization of physical properties of binary system of erythromycin stearate-sodium starch glycolate by compression force effect. Maj Farm Indones. 2011;2211(22):43-49.


Abstract Views: 225

PDF Views: 0




  • Preparation and Physical Characterization of Andrographolide-Chitosan-Alginate Microparticles prepared by Ionic Gelation–Freeze Drying

Abstract Views: 225  |  PDF Views: 0

Authors

Retno Sari
Department of Pharmaceutics, Universitas Airlangga, Dharmawangsa Dalam, Surabaya 60268, Indonesia
Sandra Tunggadewi
Department of Pharmaceutics, Universitas Airlangga, Dharmawangsa Dalam, Surabaya 60268, Indonesia
Abhimata Paramanandana
Department of Pharmaceutics, Universitas Airlangga, Dharmawangsa Dalam, Surabaya 60268, Indonesia
Helmy Yusuf
Department of Pharmaceutics, Universitas Airlangga, Dharmawangsa Dalam, Surabaya 60268, Indonesia

Abstract


This study aimed to determine the effect of alginate concentration on physical characterization (morphology, physical state, and encapsulation efficiency) of andrographolide-chitosan-alginate microparticles prepared by ionic gelation- freeze drying method. Andrographolide-chitosan-alginate microparticles were produced with various amount of alginate by ionic gelation method using tripholyphospate (TPP) as crosslinker and maltodextrin as a lyoprotectant. The physical characteristics were evaluated using Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectrophotometer (FT-IR), Differential Thermal Analyzer (DTA), Powder X-ray diffractrometer (PXRD), drug content was evaluated using High Pressure Liquid Chromatography (HPLC). The results showed that andrographolide crystallinity changed after encapsulated inside the microparticles supported by changes in IR spectra, and melting point. Furthermore, increased alginate concentration showed rougher surface morphology. The amount of alginate affected the characteristics of the andrographolide-chitosan-alginate microparticles by altering the crystallinity and lowering melting point. The highest encapsulation efficiency shown by microparticles with 0.5% alginate.

Keywords


Microparticles, Andrographolide, Chitosan, Alginates, Ionic Gelation.

References